Amyloidogenic Properties of Peptides Derived from the VHL Tumor Suppressor Protein. (6th September 2021)

• Record Type: Journal Article
• Authors: Kumar, Vijay; Viswanathan, Guru Krishna Kumar; Ralhan, Krittika; Gazit, Ehud; Segal, Daniel
• Is Part Of: ChemMedChem. Volume 16:Number 23(2021)
• Page Start: 3565
• Page End: 3568
• Publication Date: 2021-09-06
• Subjects: Amyloids -- Cancer -- Protein aggregation -- Tumor suppressor protein -- VHL protein; Pharmaceutical chemistry -- Periodicals; 615.19005
• DOI: 10.1002/cmdc.202100441
• Languages: English
• ISSNs: 1860-7179

Abstract

Abstract: The von Hippel‐Lindau tumor suppressor protein (pVHL) is involved in maintaining cellular oxygen homeostasis through the regulated degradation of HIF‐α. The intrinsically disordered nature of pVHL makes it prone to aggregation that impairs its function, and this is further aggravated in mutant versions of the protein, thus promoting tumor development. By using in silico analysis, we predicted six peptide fragments from pVHL to be amyloidogenic. This was verified for two of the peptides by biophysical approaches, which demonstrated self‐assembly and formation of β‐sheet‐rich aggregates, which, under transmission electron microscopy, atomic force microscopy, and X‐ray diffraction, displayed typical fibrillar amyloid characteristics. These motifs may serve as proxies for exploring the nature of pVHL aggregation. Abstract : Amyloid formation by tumor suppressor proteins has been suggested as a novel cause of cancer development. Here, using computational and biophysical techniques, we demonstrate that fragments derived from the aggregation‐prone pVHL tumor suppressor possess amyloidogenic characteristics.