Cd‐free InP/ZnSeS quantum dots for ultrahigh‐resolution imaging of stimulated emission depletion. Issue 12 (28th September 2021)

• Record Type: Journal Article
• Authors: Liu, Aikun; Xing, Xiuquan; Cai, Haojie; Zeng, Yutian; Guo, Jiaqing; Li, Hao; Yan, Wei; Zhou, Feifan; Song, Jun; Qu, Junle
• Is Part Of: Journal of biophotonics. Volume 14:Issue 12(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-09-28
• Subjects: Cd‐free -- InP/ZnSeS QDs -- STED nanoscopy -- ultrahigh resolution; Photonics -- Periodicals; Optical materials -- Periodicals; Optics -- Periodicals; Medical instruments and apparatus -- Periodicals; 621.3605
• DOI: 10.1002/jbio.202100230
• Languages: English
• ISSNs: 1864-063X

Abstract

Abstract: Stimulated emission depletion (STED) nanoscopy is a promising super‐resolution imaging technique for microstructure imaging; however, the performance of super‐resolution techniques critically depends on the properties of the fluorophores (photostable fluorophores) used. In this study, a suitable probe for improving the resolution of STED nanoscopy was investigated. Quantum dots (QDs) typically exhibit good photobleaching resistance characteristics. In comparison with CdSe@ZnS QDs and CsPbBr3 QDs, Cd‐free InP/ZnSeS QDs have a smaller size and exhibit an improved photobleaching resistance. Through imaging using InP/ZnSeS QDs, we achieved an ultrahigh resolution of 26.1 nm. Furthermore, we achieved a 31 nm resolution in cell experiments involving InP/ZnSeS QDs. These results indicate that Cd‐free InP/ZnSeS QDs have significant potential for application in fluorescent probes for STED nanoscopy. Abstract : The environmentally friendly InP/ZnSeS quantum dots (InP/ZnSeS QDs) was successfully synthesized by a two‐step method. The as‐prepared InP/ZnSeS QDs possess smaller size and stronger photobleaching resistance. So, the InP/ZnSeS QDs as fluorescent probes are applied in cell super‐resolution imaging. These results show that Cd‐free InP/ZnSeS QDs have significant potential for application in fluorescent probes for stimulated emission depletion nanoscopy.