A new biomimetic nanozyme of hemin/graphdiyne oxide with superior peroxidase-like activity for colorimetric bioassays. Issue 23 (8th November 2021)

• Record Type: Journal Article
• Authors: Zhu, Qiqi; Yuan, Yonghua; Yan, Bin; Zhou, Jing; Zuo, Jianli; Bai, Lijuan
• Is Part Of: Analyst. Volume 146:Issue 23(2021)
• Page Start: 7284
• Page End: 7293
• Publication Date: 2021-11-08
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/d1an01456k
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : This work describes a novel colorimetric biosensor for ultrasensitive detection of H2 O2 and glucose using a hemin/GDYO nanocomposite as a new biomimetic nanozyme with superior peroxidase-like activity. Abstract : Graphdiyne oxide (GDYO) is a novel type of two-dimensional carbon allotrope nanomaterial consisting of a large conjugated system and excellent chemical stability. To date, application of GDYO as a nanozyme in biosensing has been rarely reported. In this study, a novel ultrasensitive colorimetric bioassay was constructed using a hemin/GDYO nanocomposite (H/GDYO) as a new nanozyme with superior peroxidase-like activity for the detection of H2 O2 and glucose. It was discovered that H/GDYO exhibited 6-fold higher peroxidase-like activity than pure hemin. Catalytic kinetic analysis showed that H/GDYO had a much higher affinity for H2 O2 and glucose than that of hemin. The designed colorimetric bioassay displayed excellent sensitivity for H2 O2 and glucose detection with a wide linear range of 0.015–0.5 mM and 0.1–10 mM, respectively, while the limit of detection (LOD) was as low as 4.39 μM and 38 μM, respectively. Moreover, it was successfully applied for the analysis of H2 O2 in milk and glucose in real human serum samples with acceptable recoveries. Importantly, the developed colorimetric bioassay shows good agreement with the results obtained from a commercial blood glucose meter. We believe that the proposed method could provide a promising prospect for medical diagnosis and biotechnology.