Antioxidant activity of SSeCAHK in HepG2 cells: a selenopeptide identified from selenium-enriched soybean protein hydrolysates. Issue 54 (18th October 2021)

• Record Type: Journal Article
• Authors: Zhang, Jian; Zhang, Qiyue; Li, He; Chen, Xinwei; Liu, Wanlu; Liu, Xinqi
• Is Part Of: RSC advances. Volume 11:Issue 54(2021)
• Page Start: 33872
• Page End: 33882
• Publication Date: 2021-10-18
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/d1ra06539d
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : Se-containing antioxidative soybean peptides were isolated and identified as SSeCAHK. The SSeCAHK had protective effects against H2 O2 -induced oxidative stress in HepG2 cells and could be used as a natural food-born antioxidant. Abstract : This paper is aimed at purifying and identifying selenium (Se)-containing antioxidative peptides from Se-enriched soybean peptides (SSP). In this work, the SSP was separated into five fractions (F1 to F5). Fraction F4, displaying the highest antioxidative activity, was further separated, and sub-fractions F4-1 to F4-5 were selected for antioxidative activity evaluation using 1, 1-diphenyl-2-picrylhydrazyl (DPPH), 2, 2-azino-bis-(3-ethylbenzo-thiazoline-6-sulphonic acid)diammonium salt (ABTS), and OH − radical scavenging assays. The Se-containing antioxidative peptides with sequence Ser–SeC–Ala–His–Lys (SSeCAHK) were identified in sub-fraction F4-1 and chemically synthesized. This Se-containing pentapeptide showed a preventive effect against hydrogen peroxide (H2 O2 )-induced oxidative stress in HepG2 cells. Pretreating the cells for 2 h with SSeCAHK (0.13–0.50 mg mL −1 ) induced strong intracellular, reactive oxygen species (ROS) scavenging activity while preventing a decrease in reduced glutathione (GSH) and an increase in malondialdehyde (MDA). Therefore, SSeCAHK treatment improved H2 O2 -induced oxidative stress in HepG2 cells, demonstrating the significant potential of SSeCAHK as a natural antioxidative functional material for dietary supplementation.