Evaluation of elastin fibres in young and aged eyelids and abdominal skin using computational 3D structural analysis. Issue 4 (22nd June 2021)

• Record Type: Journal Article
• Authors: Tohgasaki, T.; Kondo, S.; Nishizawa, S.; Ishiwatari, S.; Sakurai, T.; Ishikawa, S.; Takeda, A.
• Is Part Of: Skin health and disease. Volume 1:Issue 4(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-06-22
• Subjects: Skin -- Diseases -- Periodicals; Dermatology -- Periodicals; Dermatology; Skin -- Diseases; Skin Diseases; Dermatology; Periodicals; Periodical; 616.5005
• DOI: 10.1002/ski2.58
• Languages: English
• ISSNs: 2690-442X

Abstract

Abstract: Background: Aging‐related degeneration of elastic fibres causes skin wrinkles and loss of elasticity. A correlation has been reported between dermal elastic fibre degradation and wrinkles. However, the mechanism of wrinkle formation is complex and unclear. To establish methods for treating wrinkles, it is necessary to understand the aging‐related morphological alterations underlying elastin fibre degradation or disappearance. Objectives: To image and analyse aging‐related three‐dimensional (3D) morphological alterations of elastic fibres in the eyelid and abdominal skin. Methods: Excised human eyelid and abdominal skin tissues were examined. The structure of elastic fibres in the skin tissues was examined via nuclear, tropoelastin and fibrillin‐1 immunostaining. Then, 3D imaging was performed using a confocal laser microscope and tissue decolourization technology. Images were analysed using a computational method. Results: The decolourization technology made it possible to image elastin fibres in 3D, and we devised a method for analyzing the elastin fibre structure using computational methods. It was quantitatively shown that the eyelid skin has a more complex fibrous structure than the abdomen, and the fibres became curved, shortened and thickened with age. Conclusions: We provide a novel 3D analysis method for elastin fibres and report age‐related alterations in elastin fibre structure in the human eyelid and abdominal skin. This method contributes to the understanding of elastin fibre degeneration in more detail than conventional methods. Applying this 3D analysis method to skin tissues will contribute to a better understanding of age‐related changes in fibres and to the development of novel wrinkle treatments. Abstract : Aging‐related degeneration of elastic fibers causes skin wrinkles and loss of elasticity. Here, we applied three‐dimensional imaging and a novel analysis method using immunostaining with decolorization technology and computational method to abdominal and eyelid skin tissues to evaluate aging‐related alterations in elastic fiber. We showed numerical differences in elastin fibers in the eyelids and abdominal skin excised from young and elderly patients. Hence, our analysis method contributes to the understanding of elastin fiber structure and further development of wrinkle treatments.