Physicochemical, morpho-structural, and biological characterization of polysaccharides from three Polygonatum spp. Issue 60 (24th November 2021)

• Record Type: Journal Article
• Authors: Bai, Jin-Bo; Ge, Ji-Chun; Zhang, Wang-Juan; Liu, Wang; Luo, Jian-Ping; Xu, Feng-Qing; Wu, De-Ling; Xie, Song-Zi
• Is Part Of: RSC advances. Volume 11:Issue 60(2021)
• Page Start: 37952
• Page End: 37965
• Publication Date: 2021-11-24
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/d1ra07214e
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : Three Polygonatum polysaccharides with different physicochemical properties exert distinct effects on free radical-scavenging abilities and the promotion of M1 macrophage polarization, while they have similar effects on GLP-1 secretion. Abstract : Polygonatum species, including P. cyrtonema, P. kingianum, and P. sibiricum, are edible plants with medicinal purposes, which have long been consumed as food due to their high nutritional value. In this study, polysaccharides from P. cyrtonema (PCP), P. kingianum (PKP) and P. sibiricum (PSP) were obtained, and their physicochemical properties and in vitro biological activities were investigated. Our results demonstrated that PCP, PKP, and PSP consist of major fructose and minor glucose, galacturonic acid, and galactose in different molar ratios with the molecular weights of 8.5 × 10 3 Da, 8.7 × 10 3 Da, and 1.0 × 10 4 Da, respectively. The three polysaccharides had triple-helical structures with β-d -Fruf, α-d -Glcp, α-d -Galp sugar residues, and an O -acetyl group, and displayed peak-shaped structures in different sizes. They also exhibited thermal, shear-thinning behavior and viscoelastic properties, and PCP presented the highest viscoelasticity. Moreover, they exerted strong free radical-scavenging abilities, and significant reducing capacity. PCP was the strongest, followed by PSP and then PKP. They significantly promoted the polarization of the M1 macrophage, with the effect of PCP ranking first. All three had similar effects on GLP-1 secretion. It is, therefore, necessary to identify the various roles of these three Polygonatum polysaccharides as functional agents based on their bioactivities and physicochemical properties.