An isocamphanyl-based fluorescent "turn-on" probe for highly sensitive and selective detection of Ga3+ and application in vivo and in vitro. Issue 23 (8th November 2021)

• Record Type: Journal Article
• Authors: Yang, Lijuan; Li, Mingxin; Wang, Yunyun; Zhang, Yan; Liu, Zhipeng; Ruan, Shutang; Wang, Zhonglong; Wang, Shifa
• Is Part Of: Analyst. Volume 146:Issue 23(2021)
• Page Start: 7294
• Page End: 7305
• Publication Date: 2021-11-08
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/d1an01368h
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : A novel fluorescent probe HT for highly selective and sensitive detection of Ga 3+ was successfully developed and applied in the detection of Ga 3+ in vitro and in vivo bioimaging. Abstract : A novel fluorescent probe 2-(4-(diethylamino)-2-hydroxybenzylidene)- N -(2, 3, 3-trimethylbicyclo[2.2.1]heptan-2-yl)hydrazinecarbothioamide (HT) was prepared in this study by a condensation reaction. HT has been confirmed to possess high specificity toward Ga 3+ over other metal ions (including Al 3+ and In 3+ ) via a distinct fluorescence light-up response. Moreover, HT exhibited good detection performances for Ga 3+ including high selectivity, excellent anti-interference ability, a wide working pH range, and good reversibility. The association constant and limit of detection (LOD) were calculated to be 5.34 × 10 3 M −1 and 1.18 × 10 −6 M, respectively. The detection mechanism of HT toward Ga 3+ was proposed and confirmed by 1 H NMR analysis, HRMS analysis, and DFT calculations. A simple test strip-based portable detecting device and a molecular INHIBIT logic circuit were established for improving its practical applicability. Furthermore, the desirable sensing performance of HT for Ga 3+ was successfully reconfirmed in MCF-7 cells and zebrafish.