Hairpin-bisulfite sequencing of cells exposed to decitabine documents the process of DNA demethylation. Issue 11 (2nd November 2021)

• Record Type: Journal Article
• Authors: Mayyas, Issam M; Weeks, Robert J; Day, Robert C; Magrath, Helena E; O'Connor, Karina M; Kardailsky, Olga; Hore, Timothy A; Hampton, Mark B; Morison, Ian M
• Is Part Of: Epigenetics. Volume 16:Issue 11(2021)
• Page Start: 1251
• Page End: 1259
• Publication Date: 2021-11-02
• Subjects: Demethylation -- decitabine -- dnmt1 inhibition; Epigenesis -- Periodicals; Epigenetica; 572.86505
• DOI: 10.1080/15592294.2020.1861169
• Languages: English
• ISSNs: 1559-2294

Abstract

ABSTRACT: Although the mechanism of DNA demethylating drugs has been understood for many years, the direct effect of these drugs on methylation of the complementary strands of DNA has not been formally demonstrated. By using hairpin-bisulphite sequencing, we describe the kinetics and pattern of DNA methylation following treatment of cells by the DNA methyltransferase 1 (DNMT1) inhibitor, decitabine. As expected, we demonstrate complete loss of methylation on the daughter strand following S-phase in selected densely methylated genes in synchronized Jurkat cells. Thereafter, cells showed a heterogeneous pattern of methylation reflecting replication of the unmethylated strand and restoration of methylation.