Fabrication of poly(t-butyl betaine carboxylate)-based nanoparticles and study on their in vivo biosecurity. Issue 18 (12th December 2021)

• Record Type: Journal Article
• Authors: Hou, Xueyan; Shi, Yongli; Yang, Mingbo; Yu, Shasha; Fan, Xue; Liang, Jinna; Pan, Xiaofei; Wang, Xiao
• Is Part Of: Journal of biomaterials science. Volume 32:Issue 18(2021)
• Page Start: 2387
• Page End: 2401
• Publication Date: 2021-12-12
• Subjects: NPs -- bio-security -- blood routine examination -- blood biochemistry -- physiological slides; Polymers -- Biocompatibility -- Periodicals; Biomedical materials -- Periodicals; 572.33
• DOI: 10.1080/09205063.2021.1971822
• Languages: English
• ISSNs: 0920-5063

Abstract

Abstract: The purpose of this article was to fabricate the novel poly( t -butyl betaine carboxylate)-S-S-poly (1, 3-dioxan-2-one) nanoparticles (PCB-tBU-S-S-PDI NPs) and study their in vivo biosecurity. The poly( t -butyl betaine carboxylate) (PCB-tBU) segment was conjugated to the poly(1, 3-dioxan-2-one)(PDI) moity with a disulfide bond to obtain the copolymer PCB-tBU-S-S-PDI. Hydrogen nuclear magnetic resonance ( 1 H NMR) and Fourier transform infrared spectroscopy (FTIR) spectra were applied to study the structure of PCB-tBU-S-S-PDI. The cargo-free NPs were administrated to Sprague-Dawley (SD) rats by intraperitoneal injection every 3 days for 30 days. Then, the blood routine examination, blood biochemistry, and histologic slides of rat's organs were carried out to monitor the in vivo biosecurity of cargo-free PCB-tBU-S-S-PDI NPs. 1 H NMR and FTIR spectra confirmed the successfully synthesis of PCB-tBU-S-S-PDI. The cargo-free NPs showed spherical morphology with an average of 139.8 ± 0.26 nm. The results of blood biochemistry and blood routine examination suggested that the cargo-free PCB-tBU-S-S-PDI NPs did not show any influence on the liver and renal functions of treated rats. Significantly, the physiological slides of treated rat's organs did not show any physiological and pathological changes. These phenomena suggested that the PCB-tBU-S-S-PDI NPs had good biosecurity, and it could be used as a vehicle for antineoplastic drug delivery.