Potent anti-prostate cancer immune response induced by dendritic cells transduced with recombinant adenoviruses encoding 4-1BBL combined with cytokine-induced killer cells. (January 2015)

• Record Type: Journal Article
• Authors: Youlin, Kuang; Jian, Kang; Siming, Liang; Li, Zhang; Weiyang, He; Chaodong, Liu; Xin, Gou
• Is Part Of: Immunotherapy. Volume 7:Number 1(2015:Jan.)
• Page Start: 13
• Page End: 20
• Publication Date: 2015-01
• Subjects: 4-1BBL -- cytokine-induced killer cells -- dendritic cells -- immunotherapy; Immunotherapy -- Periodicals; 615.37
• DOI: 10.2217/imt.14.92
• Languages: English
• ISSNs: 1750-743X

Abstract

Aim: To test the effect of dendritic cells (DCs) transduced with recombinant adenoviruses encoding 4-1BBL combined with cytokine-induced killer cells (CIKs) against prostate cancer.Method: Flow cytometry was used to detect the surface markers of the co-cultured cells, and cytotoxicity against prostate cancer cells in vitro as well as antitumor activities in vivo were observed.Results: Our results showed that Ad-4-1BBL-transduced DCs could increase percentage of CD3 + CD56 + cells in CIKs, and CIKs co-cultured with Ad-4-1BBL-transduced DCs could augment the secretion of IL-12 and IFN-γ and decrease TGF-β production. In addition, Ad-4-1BBL-transduced DCs enhanced the cytotoxicity of CIKs against prostate cancer and resulted in inhibition of tumor growth and tumor-bearing animals' survival.Conclusion: These results demonstrate that 4-1BBL-engineered DCs can improve CIKs cytotoxicity against prostate cancer cells.