Depyrogenation using plasmas: A novel approach for endotoxin deactivation using a dielectric barrier discharge at atmospheric pressure. Issue 11 (7th September 2021)

• Record Type: Journal Article
• Authors: Bhatt, Naman; Brier‐Jones, Justin; Trosan, Duncan; Brinkley, Cade; Pecoraro, Joshua; Smallwood, Jann; Crofton, Andrew; Hudson, Samuel; Kirsch, Wolff; Stapelmann, Katharina; Shannon, Steven
• Is Part Of: Plasma processes and polymers. Volume 18:Issue 11(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-09-07
• Subjects: atmospheric pressure plasmas -- chitosan -- depyrogenation -- endotoxin deactivation -- low‐temperature plasmas; Plasma polymerization -- Periodicals; Plasma-enhanced chemical vapor deposition -- Periodicals; Plasma chemistry -- Periodicals
• DOI: 10.1002/ppap.202100089
• Languages: English
• ISSNs: 1612-8850

Abstract

Abstract : Plasma treatment inside a hermetically sealed bag. The setup allows a sealed environment to treat delicate materials with different plasmas while avoiding cross‐contamination issues. This also allows the bags to be transported after the treatments. Abstract: Developing a low‐cost depyrogenation process is vital in extending the medical applicability of polymers that can be used in medicine. We present an overview of the plasma‐based depyrogenation literature and address the need to develop a nonthermal plasma‐based depyrogenation process for delicate materials such as chitosan. We present a low‐cost plasma apparatus to treat chitosan powder in hermetically sealed bags. We decouple the experiments into two parts: depyrogenation experiments for dried standard endotoxin on glass slides and analysis of chitosan modifications through Fourier‐transform infrared spectroscopy. We demonstrate depyrogenation efficacy with up to a 4‐log reduction in endotoxin levels and discuss minor changes observed in plasma‐treated chitosan.