Isoflurane induces Art2‐Rsp5‐dependent endocytosis of Bap2 in yeast. Issue 11 (29th September 2021)

• Record Type: Journal Article
• Authors: Kozu, Fumi; Shirahama‐Noda, Kanae; Araki, Yasuhiro; Kira, Shintaro; Niwa, Hitoshi; Noda, Takeshi
• Is Part Of: FEBS open bio. Volume 11:Issue 11(2021)
• Page Start: 3090
• Page End: 3100
• Publication Date: 2021-09-29
• Subjects: anesthetic -- arrestin -- endocytosis -- transporter -- ubiquitin; Molecular biology -- Periodicals; Cytology -- Periodicals; Life sciences -- Periodicals; Biological Science Disciplines -- Periodicals; Molecular Biology -- Periodicals; Cell Biology -- Periodicals; Cytology; Life sciences; Molecular biology; Periodicals; 572.805
• DOI: 10.1002/2211-5463.13302
• Languages: English
• ISSNs: 2211-5463

Abstract

Abstract : Although general anesthesia is indispensable during modern surgical procedures, the mechanism by which inhalation anesthetics act on the synaptic membrane at the molecular and cellular level is largely unknown. In this study, we used yeast cells to examine the effect of isoflurane, an inhalation anesthetic, on membrane proteins. Bap2, an amino acid transporter localized on the plasma membrane, was endocytosed when yeast cells were treated with isoflurane. Depletion of RSP5, an E3 ligase, prevented this endocytosis and Bap2 was ubiquitinated in response to isoflurane, indicating an ubiquitin‐dependent process. Screening all the Rsp5 binding adaptors showed that Art2 plays a central role in this process. These results suggest that isoflurane affects Bap2 via an Art2‐Rsp5‐dependent ubiquitination system. Abstract : Bap2, an amino acid transporter localized on the plasma membrane in yeast, was endocytosed when yeast cells were treated with isoflurane, general anesthesia. Depletion of RSP5, an E3 ligase, and Art2 (arrestin) prevented this endocytosis and Bap2. Thus, isoflurane affects Bap2 via an Art2‐Rsp5‐dependent ubiquitination system.