Get ready for the CRISPR/Cas system: A beginner's guide to the engineering and design of guide RNAs. (28th July 2021)

• Record Type: Journal Article
• Authors: Ma, Shufeng; Lv, Jie; Feng, Zinan; Rong, Zhili; Lin, Ying
• Is Part Of: Journal of gene medicine. Volume 23:Number 11(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-07-28
• Subjects: CRISPR/Cas9 -- CRISPR/Cas9 delivery -- gene editing -- guide RNA -- guide RNA engineering; Genetic transformation -- Periodicals; Gene Transfer -- Periodicals; Gene Therapy -- Periodicals; 616.042
• DOI: 10.1002/jgm.3377
• Languages: English
• ISSNs: 1099-498X

Abstract

Abstract: The clustered regularly interspaced short palindromic repeats (CRISPR) system is a state‐of‐the‐art tool for versatile genome editing that has advanced basic research dramatically, with great potential for clinic applications. The system consists of two key molecules: a CRISPR‐associated (Cas) effector nuclease and a single guide RNA. The simplicity of the system has enabled the development of a wide spectrum of derivative methods. Almost any laboratory can utilize these methods, although new users may initially be confused when faced with the potentially overwhelming abundance of choices. Cas nucleases and their engineering have been systematically reviewed previously. In the present review, we discuss single guide RNA engineering and design strategies that facilitate more efficient, more specific and safer gene editing. Abstract : In the CRISPR/Cas system, guide RNA (gRNA) plays an essential role in target site recognition and binding. The gRNA should be well‐designed for a specific research purpose. Engineered gRNAs can improve gene‐editing efficiency and specificity, as well as broaden CRISPR applications in both basic and clinical research.