Long non-coding RNA PROX1-AS1 knockdown upregulates microRNA-519d-3p to promote chemosensitivity of retinoblastoma cells via targeting SOX2. Issue 20 (18th October 2021)

• Record Type: Journal Article
• Authors: Chen, Yanyan; Lu, Boyang; Liu, Lei; Pan, Xuefeng; Jiang, Chunying; Xu, Hui
• Is Part Of: Cell cycle. Volume 20:Issue 20(2021)
• Page Start: 2149
• Page End: 2159
• Publication Date: 2021-10-18
• Subjects: Retinoblastoma -- long non-coding rna prox1-antisense rna1 -- microrna-519d-3p -- sex determining region y-box 2 -- proliferation -- apoptosis; Cell cycle -- Periodicals; 571.84377
• DOI: 10.1080/15384101.2021.1971352
• Languages: English
• ISSNs: 1538-4101

Abstract

ABSTRACT: Objective: Long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) participate in tumor progression, while the role of PROX1-antisense RNA1 (PROX1-AS1) sponging miR-519d-3p in retinoblastoma (RB) remains largely unknown. We aim to explore the effect of the PROX1-AS1/miR-519d-3p/sex determining region Y-box 2 (SOX2) in chemosensitivity of RB cells. Methods: Expression of PROX1-AS1, miR-519d-3p and SOX2 in RB tissues and cells was determined. The drug-resistant cell lines were established and respectively intervened with PROX1-AS1 or miR-519d-3p expression to explore their roles in drug resistance and malignant behaviors of the drug-resistant cells. The binding relationships between PROX1-AS1 and miR-519d-3p, and between miR-519d-3p and SOX2 were evaluated. Results: PROX1-AS1 and SOX2 were upregulated while miR-519d-3p was downregulated in RB tissues and cells, especially in drug-resistant cells. The PROX1-AS1 inhibition or miR-519d-3p elevation suppressed the drug resistance, proliferation, migration and invasion, and promoted apoptosis of the drug-resistant RB cells. Moreover, PROX1-AS1 sponged miR-519d-3p and miR-519d-3p targeted SOX2. Conclusion: PROX1-AS1 knockdown upregulates miR-519d-3p to promote chemosensitivity of RB cells via targeting SOX2.