Hsa_circ_0060937 accelerates non-small cell lung cancer progression via modulating miR-195-5p/HMGB3 pathway. Issue 19 (2nd October 2021)

• Record Type: Journal Article
• Authors: Xi, Junfeng; Xi, Yunfeng; Zhang, Zhibin; Hao, Yanhong; Wu, Fei; Bian, Burong; Hao, Guangjun; Li, Weiwei; Zhang, Shuqun
• Is Part Of: Cell cycle. Volume 20:Issue 19(2021)
• Page Start: 2040
• Page End: 2052
• Publication Date: 2021-10-02
• Subjects: Circ_0060937 -- miR-195-5p -- HMGB3 -- glycolysis -- non-small cell lung cancer; Cell cycle -- Periodicals; 571.84377
• DOI: 10.1080/15384101.2021.1969203
• Languages: English
• ISSNs: 1538-4101

Abstract

ABSTRACT: Circular RNAs (circRNAs) exert a critical effect on tumorigenesis and development. Our research aimed to clarify the function and underlying mechanism of circ_0060937 inNSCLC. The concentrations of circ_0060937, miR-195-5p and high-mobility group box 3 (HMGB3) were monitored via qRT-PCR and western blot assays. Additionally, cell proliferation, apoptosis, migration and invasion were assessed using CCK-8, colony formation, flow cytometry and transwell assays. Glycolysis was evaluated via detecting glucose uptake and lactate product. The association between miR-195-5p and circ_0060937/HMGB3 were validated using dual-luciferase reporter, RNA pull-down and RIP assays. Furthermore, in vivo experiment was performed to analyze tumorigenesis.Circ_0060937 and HMGB3 levels were elevated, whereas miR-195-5p level was dropped in NSCLC. Circ_0060937 down-regulation restrainedNSCLC cell proliferation, migration, invasion and glycolysis, and triggered apoptosis. Knockdown of circ_0060937 restrained NSCLC development via absorbing miR-195-5p. Circ_0060937 silencing inhibited NSCLC progression by mediating HMGB3. Besides, circ_0060937 depletion suppressed tumor growth in vivo .Circ_0060937 knockdown hindered NSCLC development and glycolysis via regulating miR-195-5p/HMGB3 pathway.