A sulfonate-based polypeptide toward infection-resistant coatings. (25th August 2021)

• Record Type: Journal Article
• Authors: Xue, Ruizhong; Zhang, Xu; Wei, Yuansong; Zhao, Ziyin; Liu, Hao; Yang, Fangping; Yin, Lichen; Song, Ziyuan; Luan, Shifang; Tang, Haoyu
• Is Part Of: Biomaterials science. Volume 9:Number 19(2021)
• Page Start: 6425
• Page End: 6433
• Publication Date: 2021-08-25
• Subjects: Biomedical materials -- Periodicals; 610.28
• DOI: 10.1039/d1bm00951f
• Languages: English
• ISSNs: 2047-4830

Abstract

Abstract : We developed a mono-component and sulfonate-based polypeptide coating with reversible on-demand bactericidal and antifouling properties. Abstract : Multifunctional coatings have gained significant attention for their promising potential to address the issue of medical device-related infections. However, they usually have multiple components in one layer which decreases the density of functional groups on surfaces and hence reduces the biological properties. Herein, we report a mono-component and sulfonate-based anionic polypeptide coating with on-demand antibacterial activity, antifouling property, and biocompatibility. The anionic polypeptide was prepared by ring-opening polymerization of l -cysteine-based N -carboxyanhydride (NCA) with allyl groups and a subsequent thiol–ene reaction to incorporate the sulfonate pendants. It adopted a 17.1–19.5% β-sheet conformation and self-assembled into a spherical nanoparticle. The polypeptide coating showed excellent in vitro antibacterial activity against both Gram-positive ( i.e., S. aureus ) and Gram-negative bacteria ( i.e., E. coli ) with >99% killing efficacy after acidic solution treatment and prominent antifouling property and biocompatibility after weak base treatment. An in vivo study revealed that the sulfonate-based polypeptide-coated polydimethylsiloxane (PDMS) exhibited good anti-infection property and histocompatibility.