AB0174 CLINICAL SIGNIFICANCE OF THE URINARY CD11C+ MYELOID CELLS IN THE PATIENTS WITH LUPUS NEPHRITIS. (June 2019)

• Record Type: Journal Article
• Authors: Kim, Jihye; Jung, Jae Hyung; Lee, Jung Sun; Oh, Ji Seon; Seo, Wook Jang; Kim, Yong-Gil; Lee, Chang-Keun; Yoo, Bin; Hong, Seokchan
• Is Part Of: Annals of the rheumatic diseases. Volume 78(2019)Supplement 2
• Page Start: 1545
• Page End: 1546
• Publication Date: 2019-06
• Subjects: Rheumatism -- Periodicals; 616.723005
• DOI: 10.1136/annrheumdis-2019-eular.2528
• Languages: English
• ISSNs: 0003-4967

Abstract

Abstract : Background: It is known that various immune cell populations play important pathogenic roles in lupus nephritis (LN). Kidney-infiltrating myeloid cells are responsible for the pathology in murine model for LN. However, the characteristics of myeloid cells in patients with LN have not previously been investigated because of difficulties of access to affected tissue. Recent studies have reported that the urine has become an alternative substitute for kidney tissue to study renal pathology. Objectives: Therefore, we investigated the characteristics and functions of urinary myeloid cells in patients with LN. Methods: Within viable CD45+ cells, the frequency and absolute numbers of myeloid cells in the urine were examined after exclusion of lymphoid cells by expression of CD3, CD19 and CD56. Expression of phenotypic markers and production of pro-inflammatory cytokines were analyzed in urinary myeloid cells of patients with LN. Results: Myeloid cells were identified by the expression of CD45+ and CD11c+ in the urine of patients with LN. The frequency and absolute numbers of myeloid cells were markedly increased in the urine of patients with proliferative LN than non-proliferative LN. In addition, titers of anti-dsDNA antibodies were correlated with the frequency or numbers of urinary CD11c+ myeloid cells. These urinary CD11c+ myeloid cells showed the phenotypes of infiltrated monocyte-derived cells rather than tissue-resident macrophage. In addition, CD11c+ myeloid cells were localized in tubulointerstitium and had capacity to produce pro-inflammatory cytokines including IL-6. Further, we found that there was a significant population of tubule cells in the urine, which is correlated with the frequency of CD11c+ myeloid cells. Conclusion: Our results indicate that CD11c+ myeloid cells are present in the urine and contribute to tubulointerstitial inflammation in proliferative LN. Disclosure of Interests: None declared