Applications of upconversion nanoparticles in cellular optogenetics. (November 2021)

• Record Type: Journal Article
• Authors: Lin, Yinyan; Yao, Yuanfa; Zhang, Wanmei; Fang, Qiuyu; Zhang, Luhao; Zhang, Yan; Xu, Yingke
• Is Part Of: Acta biomaterialia. Volume 135(2021)
• Page Start: 1
• Page End: 12
• Publication Date: 2021-11
• Subjects: Upconversion -- Nanoparticles -- Near-infrared -- Optogenetics; UCNPs upconversion nanoparticles -- μ-LEDs micro light-emitting diodes -- NIR near-infrared -- UV ultraviolet -- ChR1/2 channelrhodopsin1/2 -- OptoXR a chimera between vertebrate rhodopsin and G protein-coupled receptor -- BR Halobacterium salinarum -- Mac Leptosphaeria maculans -- Arch archaerhodopsin-3 from Halorubrum sodomense -- ArchT an archaerhodopsin from Halorubrum strain TP009 -- CRY2 cryptochromes 2 -- CIB1 cryptochrome-interacting basic-helix-loop-helix -- CRY2(E490G) CRY2clust, CRY2 variants -- LOV light-oxygen-voltage -- pdDronpas photodissociable Dronpa -- CBDs bacterial cobalamin (vitamin B12) binding domains -- mVChR1 modified Volvox channelrhodopsin-1 -- C1V1 a variant generated by fusing the N-terminal sequence of ChR1 with the C-terminal sequence of Volvox-Channelrhodopsin-1 -- HRs halorhodopsins -- ACRs channelrhodopsin variant anion channelrhodopsins -- PsChR Platymonas subcordiformis -- ReaChR red-activatable ChR -- CnChR1 a red-shifted channelrhodopsin from the species Chlamydomonas noctigama -- ChRs channelrhodopsins -- NpHR Natronomonas pharaonis -- HsHR halorhodopsin from Natronomonas pharaonis -- LOVs LOV domains -- PAS Period-ARNT-Singleminded -- AsLOV2 Avena sativa phototropin 1 -- VVD Vivid -- YtvA Bacillus subtilis stress response protein -- FixL Bradyrhizobium japonicum -- YF1 the histidine kinase -- FixJ the cognate, noncovalently bound, response regulator of FixL -- pDawn pDusk, plasmids -- FixK2 a promoter -- IFN-γ interferon-γ -- ESA excited-state absorption -- PA photon avalanche -- ETU energy transfer upconversion -- CS cooperative sensitization -- EMU energy migration upconversion -- GSA ground state absorption -- LNPs lanthanide nanoparticles -- VTA the ventral tegmental area -- MS the medial septum -- LMPs lanthanide micro-particles -- dSTR the dorsal striatum -- mPFC medial prefrontal cortex -- wM1 whisker motor cortex -- MRI magnetic resonance imaging -- C. elegans Caenorhabditis elegans -- miniSOG the mini singlet oxygen generator -- PMMA poly(methyl methacrylate) -- PDMS polydimethylsiloxane -- FADD Fas-associating protein with a novel death domain -- PEI poly(ethylene imine) -- UCRs upconversion rods -- STIM1 stromal interaction molecule 1, CRAC, a Ca2+ release-activated Ca2+ channel -- PMA phorbol 12-myristate 13-acetate -- MHC the major histocompatibility complex -- CCR7 CC-chemokine receptor 7 -- UCNPs-Stv streptavidin-conjugated UCNPs -- StrepTag streptavidin-binding tag -- ORAI1 calcium release-activated calcium modulator 1 -- V5-ChR2m the extracellular N-terminal of ChR2 tagged with the V5 epitope -- NAv-UCNPs the surface of UCNPs functionalized with NeutrAvidin -- FAs folic acids -- quasi-CW quasi-continuous wave -- DIO open reading frame -- BAC bacterial artificial chromosome -- NSCs neural stem cells; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1016/j.actbio.2021.08.035
• Languages: English
• ISSNs: 1742-7061

Abstract

Abstract: Upconversion-mediated optogenetics is an emerging powerful technique to remotely control and manipulate the deep-tissue protein functions and signaling pathway activation. This technique uses lanthanide upconversion nanoparticles (UCNPs) as light transducers and through near-infrared light to indirectly activate the traditional optogenetic proteins. With the merits of high spatiotemporal resolution and minimal invasiveness, this technique enables cell-type specific manipulation of cellular activities in deep tissues as well as in living animals. In this review, we introduce the latest development of optogenetic modules and UCNPs, with emphasis on the integration of UCNPs with cellular optogenetics and their biomedical applications on the control of neural/brain activity, cancer therapy and cardiac optogenetics in vivo . Furthermore, we analyze the current developed strategies to optimize and advance the upconversion-mediated optogenetics and discuss the remaining challenges of its further applications in biomedical study and clinical translational research. Statement of significance: Optogenetics harnesses photoactivatable proteins to optically stimulate and control intracellular activities. UCNPs-mediated NIR-activatable optogenetics uses lanthanide upconversion nanoparticles (UCNPs) as light transducers and utilizes near-infrared (NIR) light to indirectly activate the traditional optogenetic proteins. The integration of UCNPs with cellular optogenetics has showed great promise in biomedical applications in regulating neural/brain activity, cancer therapy and cardiac optogenetics in vivo . The evolution and optimization of functional UCNPs and the discovery and engineering of novel optogenetic modules would both contribute to the advance of such unique hybrid technology, which may lead to discoveries in biomedical research and provide new treatments for human diseases. Graphical abstract: Image, graphical abstract