Characterization of biofilm formation and reduction of hexavalent chromium by bacteria isolated from tannery sludge. (January 2022)

• Record Type: Journal Article
• Authors: Maurya, Annapurna; Kumar, P. Senthil; Raj, Abhay
• Is Part Of: Chemosphere. Volume 286:Part 2(2022)
• Publication Date: 2022-01
• Subjects: Tannery effluent sludge -- Biofilm-forming bacteria -- Planktonic cells -- SEM -- ATR-FTIR -- Chromium removal; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2021.131795
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: Biofilm formation ability of bacteria makes them potential in the field of tannery effluent treatment. However, the hazardous nature of effluent and environmental conditions may disturb the biofilm formation ability of bacteria which ultimately affects their effluent treatment efficiency. Accordingly, we isolated and characterized biofilm-forming bacteria Bacillus vallismortis (MT027009), Bacillus haynesii (MT027008), and Alcaligenes aquatilis (MT027005) from tannery sludge and examined them for biofilm formation under variable environmental conditions. Biofilm formation in tryptic soy broth (TSB) at different incubation times (24–120 h) revealed that the biofilm formation activity of the strain B. haynesii was not affected by incubation time, whereas the increase in biofilm formation was observed in the case of B. vallismortis (28 %) and A. aquatilis (52 %) after 48 h. The medium pH (pH 5.0–9.0) had a limited effect on biofilm formation except in the case of A. aquatilis at pH 5.0 (94 %) and pH 9.0 (80 %). Furthermore, compared to the controls (only TSB), the strains B. vallismortis, B. haynesii, and A. aquatilis showed enhanced biofilm formation in undiluted tannery effluent (28, 33, and 21 %) and 25 mg L −1 Cr(VI) (23 %, 48 % 32 %). The biofilm structure was influenced by Cr(VI) as revealed by scanning electron microscopy (SEM) analysis. The results of Cr(VI) bioreduction studies suggest that bacterial biofilm (60–99 %) has a greater potential to remove Cr(VI) than planktonic cells (43–94 %). The results of the study provide important data on biofilm formation by indigenous bacteria in effluent environment conditions, making them potential isolates for tannery effluent treatment. Graphical abstract: Image 1 Highlights: Formation of biofilm under mixed culture conditions. Tannery effluent and Cr(VI) induced biofilm formation from 20 to 62 %. Biofilm formation and Cr(VI) reduction were maximum by B. haynesii . Cr(VI) reduction was 60–99 % from bacterial biofilm.