Novel dual labelled nanoprobes for nanosafety studies: Quantification and imaging experiment of CuO nanoparticles in C. elegans. (January 2022)

• Record Type: Journal Article
• Authors: Butreddy, Pravalika; Chakraborty, Swaroop; Soppina, Pushpanjali; Behera, Rakesh; Soppina, Virupakshi; Misra, Superb K.
• Is Part Of: Chemosphere. Volume 286:Part 2(2022)
• Publication Date: 2022-01
• Subjects: Stable isotope -- Fluorescent labelling -- Nanosafety -- CuO nanoparticles -- Tracing and imaging -- Fate and accumulation; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2021.131698
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: Metal oxide nanoparticles have been extensively studied for their toxicological impacts. However, accurate tracing/quantification of the nanomaterials and their biological responses are difficult to measure at low concentrations. To overcome the challenge, we developed a dual-labelling technique of CuO nanoparticles with a stable isotope of 65 Cu, and with rhodamine dye. In vivo experiments on C. elegans were performed using natural feeding of Rhodamine B isothiocyanate-(3 aminopropyl) triethoxysilane functionalized 65 CuO nanoprobes (RBITC-APTES@ 65 CuO) (size = 7.41 ± 1 nm) within the range of Predicted Environmental Concentration (PEC) of CuO nanoparticles in soil and sediments. Fluorescence emission (570 nm) was detected in the lumen of the intestine and the pharynx of C. elegans with no impact of nanoparticle exposure on the brood size and life span of worms. The ingested fluorescent labelled RBITC-APTES@ 65 CuO nanoprobes did not enter the reproductive system and were distributed in the alimentary canal of C. elegans . Strong fluorescent signals from the ingested RBITC-APTES@ 65 CuO nanoprobes were achieved even after 24 h of exposure demonstrating the high stability of these nanoprobes in vivo . The net accumulation measured of 65 Cu in C. elegans after background subtraction was 0.001 μg mg −1 (3.52 %), 0.005 μg mg −1 (1.76 %) and 0.024 μg mg −1 (1.69 %) for an exposure concentration of 0.0284 μg mg −1, 0.284 μg mg −1, and 1.42 μg mg −1 of 65 Cu, respectively. Using C. elegans as a model organism, we demonstrated that RBITC-APTES tagged 65 CuO nanoparticles acted as novel nanoprobes for measuring the uptake, accumulation, and biodistribution through quantification and imaging the nanoprobes at a very low exposure concentration ( 65 CuO concentration: 0.033 μg mg −1 ). Graphical abstract: Image 1 Highlights: Synthesis of 65 Cu isotopically enriched and Rhodamine B dye labelled dual nanoprobes. Nanoprobes fed naturally to C. elegans at Predicted Environmental Concentration (PEC) of CuO nanoparticles. No observed reduction in life span and brood size of worms. Precise quantification and imaging of nanoprobes in vivo at a very low concentration 0.0284 μg mg −1 . Nanoprobes are chemically stable in vivo even after 48 h of exposure.