Chitinophaga rhizophila sp. nov., isolated from rhizosphere soil of banana. Issue 11 (30th November 2021)

• Record Type: Journal Article
• Authors: Zhang, Xian-Jiao; Feng, Guang-Da; Yang, Fan; Zhu, Honghui; Yao, Qing
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 71:Issue 11(2021)
• Publication Date: 2021-11-30
• Subjects: Chitinophaga -- phylogeny -- rhizosphere -- taxonomy; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.005118
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A Gram-stain-negative, aerobic and rod-shaped bacterium, designated as strain B61 T, was isolated from rhizosphere soil of banana collected from Dongguan, Guangdong Province, PR China. Growth occurred at 15–40 °C, within a pH range of pH 6.0–9.0. Results of 16S rRNA gene sequence similarity and phylogenetic analyses showed that strain B61 T was most closely related to ' Chitinophaga agri ' KACC 21303 (98.9 %) and Chitinophaga pinensis DSM 2588 T (98.8 %). The genome size was 7.6 Mb with a G+C content of 45.2 mol%. The genome-inferred average nucleotide identity values between strain B61 T and two closely related strains were 79.2 and 79.3 %, respectively, with corresponding digital DNA–DNA hybridization values of 22.3 and 22.6 %. The major fatty acids of the novel strain were iso-C15:0, C16:1 ω 5 c and iso-C17:0 3-OH and the sole respiratory quinone was menaquinone 7 (MK-7). The polar lipids consisted of phosphatidylethanolamine, five unidentified aminolipids, four unidentified glycolipids and six unidentified lipids. The phenotypic and phylogenetic results clearly supported that strain B61 T represents a novel species of the genus Chitinophaga, for which the name Chitinophaga rhizophila, sp. nov. is proposed, with the type strain B61 T (=GDMCC 1.2608 T =KCTC 82856 T ).