Molecular and functional characterization of MST2 in grass carp during bacterial infection. Issue 119 (December 2021)

• Record Type: Journal Article
• Authors: Li, Yanan; Qiu, Xiaolong; Lu, Zhijie; Zhan, Fanbin; Yang, Minxuan; Sarath Babu, V.; Li, Jun; Qin, Zhendong; Lin, Li
• Is Part Of: Fish & shellfish immunology. Issue 119(2021)
• Page Start: 19
• Page End: 30
• Publication Date: 2021-12
• Subjects: MST2 -- Ctenopharyngodon idella -- Innate immunity -- Antioxidant regulator; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2021.09.026
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: The regulation of host redox homeostasis is critically important in the immune response to pathogens. The "mammalian sterile 20-like" kinase 2 (MST2) has been shown to play a role in apoptosis, cell proliferation, and cancer; however, few studies have examined its ability to modulate redox homeostasis during innate immunity, especially in teleost fish. In this study, we cloned the MST2 gene of Ctenopharyngodon idella ( Ci MST2) and analyzed its tissue distribution. Ci MST2 was present in most of the studied tissues, and it was most highly expressed in brain tissue. Expression patterns analysis revealed that MST2 mRNA and protein were significantly up-regulated under bacterial infection, suggesting that it is involved in the immune response. Bacterial stimulation significantly increased the level of antioxidases. To explore the interplay between Ci MST2 and antioxidant regulation, we examined the effects of Ci MST2 overexpression and conducted RNA interference assays in vitro . Ci MST2 overexpression significantly increased the expression levels of nuclear factor E2-related factor 2 (Nrf2) and other antioxidases and vice versa, revealing that Ci MST2 regulated host redox homeostasis via Nrf2-antioxidant responsive element (ARE) signaling. Overall, our findings provide a new perspective on the role of MST2 in innate immunity in teleosts as well as insights that will aid the prevention and control of disease in the grass carp farming industry. Highlights: 1. Mst2 widely existed in all tested tissues in healthy grass carp. 2. The bacterial infection significantly up-regulated the expression of Ci Mst2. 3. The bacterial infection significantly up-regulated the antioxidant system of grass carp. 4. Ci Mst2 plays a vital antioxidant regulator in antibacterial immunity through Nrf2-ARE signaling.