Identification and genetic characterization of Contracaecum sp. (Nematoda: Anisakidae) from China. Issue 6 (10th August 2020)

• Record Type: Journal Article
• Authors: ZHANG, Qingxun; MENG, Meng; HUANG, Chengmei; FENG, Shengyong; LIU, Jie; TANG, Yan; FAN, Yu; YUAN, Guohui; HAN, Shuyi; LUO, Jing; ZHAO, Baohua; HE, Hongxuan
• Is Part Of: Integrative zoology. Volume 16:Issue 6(2021)
• Page Start: 929
• Page End: 938
• Publication Date: 2020-08-10
• Subjects: Anisakid -- epidemiology -- morphology -- phylogenetic analysis; Zoology -- Periodicals; Animals -- Periodicals; Zoologie -- Périodiques; Animaux -- Périodiques; 590.5
• DOI: 10.1111/1749-4877.12465
• Languages: English
• ISSNs: 1749-4869

Abstract

Abstract: Contracaecum species are economically important fish‐borne larval nematodes with zoonotic significance. In June 2019, more than 100 piscivorous birds died in their habitats close to the Wild Duck Lake, located in Yanqing, northwest of Beijing, China. Post‐mortem examination of Black Night Herons ( Nycticorax ) revealed the presence of numerous anisakid nematodes in the proventriculus. Recovered nematodes were identified as Contracaecum sp. based on morphological description. Phylogenetic analysis of the mitochondrial (mt) genome and the ITS gene showed that sequences of Contracaecum sp. Beijing isolates were grouped into a new individual cluster. Furthermore, the parasite was successfully isolated from fresh dead birds, feces of piscivorous birds, and fish and prevalence ranged from 8.0% to 81.8%. Consequently, our study demonstrated Contracaecum sp. infections in different sources from China, which might constitute a threat to wildlife, aquaculture, and public health. Abstract : Recovered nematodes were identified as Contracaecum sp. based on morphological and etiology analysis. Epidemiological investigation showed that the infection rates ranged from 8.0% to 81.8% in fresh dead birds, feces of piscivorous birds, and fish. Phylogenetic analysis of the mitochondrial genome showed that sequences of Contracaecum sp. Beijing isolates were grouped into a new individual cluster.