An oxygenated perfluorocarbon emulsion improves liver graft preservation evaluated in DCD livers of male sprague dawley rats. (8th September 2021)

• Record Type: Journal Article
• Authors: Wu, Guoyi; Liu, Yu; Rui, Chen; Zhan, Shanshan; Wang, Jun; Cai, Shizhen; Shi, Xiaolei; Ding, Yitao
• Is Part Of: Transplant international. Volume 34:Number 11(2021)
• Page Start: 2087
• Page End: 2097
• Publication Date: 2021-09-08
• Subjects: emulsion -- liver transplantation -- oxygenated preservation -- perfluorocarbon; Transplantation of organs, tissues, etc -- Periodicals; 617.95405
• DOI: 10.1111/tri.13996
• Languages: English
• ISSNs: 0934-0874

Abstract

Summary: The application of perfluorocarbons, which can carry large quantities of oxygen, in organ preservation was limited by their poor solubility in water. A stable form of perfluorocarbon dispersed in suitable buffers is urgently needed. Perfluorocarbon emulsion was designed and characterized with respect to size distribution, rheology, stability, and oxygen‐carrying capacity. The state of DCD rat donor livers preserved by the oxygenated perfluorocarbon emulsion was studied after ex vivo reperfusion by using biochemistry, pathology, and immunohistochemistry methods. Perfluorocarbon emulsion was successfully prepared by high‐pressure homogenization. Optimized perfluorocarbon emulsion showed nanoscale size distribution, good stability, and higher oxygen loading capacity than that of HTK solution or water. The state of preserved livers after cardiac death rat liver was improved significantly after static cold storage for 48 hours in this oxygenated perfluorocarbon emulsion. The ATP content and down‐regulation of HIF‐1a expression after preservation of the liver graft by the oxygenated perfluorocarbon emulsion suggested the advantage of adequate oxygen supply for adequate time. This perfluorocarbon emulsion reported here might be considered a promising system for oxygenated donor liver storage by attenuation of hypoxia. Abstract : A perfluorocarbon emulsion was designed for oxygenated preservation of liver grafts in organ transplantation based on the oxygenation capability of perfluorocarbon. Preserved with the oxygenated perfluorocarbon emulsion, the improvement of DCD rat donor livers was observed with higher ATP content and down‐regulated HIF‐1a expression.