Modular assembly and encoding strategies for dual-display DNA-encoded chemical libraries. Issue 92 (3rd November 2021)

• Record Type: Journal Article
• Authors: Oehler, Sebastian; Plais, Louise; Bassi, Gabriele; Neri, Dario; Scheuermann, Jörg
• Is Part Of: Chemical communications. Volume 57:Issue 92(2021)
• Page Start: 12289
• Page End: 12292
• Publication Date: 2021-11-03
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/d1cc04306d
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : Modular assembly methodologies of DNA-encoded chemical libraries featuring dual-display of organic molecules. Abstract : DNA-encoded chemical libraries (DELs) are increasingly being used for the discovery of protein ligands and can be constructed displaying either one or two molecules at the extremities of the two complementary DNA strands. Here, we describe that DELs, featuring the simultaneous display of two molecules, can be encoded using various types of DNA structures, which go beyond the use of conventional double-stranded DNA fragments. Specifically, we compared dual-display methodologies in hairpin, circular or linear formats in terms of polymerase chain reaction (PCR) amplifiability and performance in affinity capture selections. The methods reported in this article highlight the feasibility and modularity of the described encoding strategies and may thus further expand the scope of DNA-encoded chemistry, particularly for the identification of compounds which recognize adjacent epitopes on the surface of target proteins of interest.