New triterpenes from Cimicifuga yunnanensis down-regulating the mRNA expression of CD147, MMP-2, and MMP-9. Issue 58 (17th November 2021)

• Record Type: Journal Article
• Authors: Lu, Ni-Hong; Li, Jie; Yang, Yong-Rui; Liu, Hong-Lu; Du, Ying-Rong
• Is Part Of: RSC advances. Volume 11:Issue 58(2021)
• Page Start: 36978
• Page End: 36988
• Publication Date: 2021-11-17
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/d1ra07828c
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : Eleven new 9, 19-cycloartane triterpenes (CTs) (1–9, 11–12 ) and one undescribed lanostane-type aglycone (10 ) were identified from Cimicifuga yunnanensis . CTs aglycones 7 and 8 potently down-regulated the mRNA expression of CD147, MMP-2, and MMP-9. Abstract : Eleven new 9, 19-cycloartane triterpenes (1–9, 11–12 ) and one undescribed lanostane-type aglycone (10 ) were identified from the aerial parts of Cimicifuga yunnanensis . The new structures were elucidated by analysis of spectroscopic data. Compounds 3–5, 7–9, and 11, without obvious cytotoxicity at 50 μM, were evaluated for inhibiting the mRNA expressions of atherosclerosis-related factors of CD147 (extracellular matrix metalloproteinase inducer, EMMPRIN), matrix metalloproteinase 2 (MMP-2) and MMP-9 in phorbol-12-myristate-13-acetate (PMA) induced Human monocytic THP-1 cells by using a quantitative real-time PCR method (q-PCR). Among them, aglycones 7 and 8 showed potent activities, whereas all tested glycosides were inactive. Compounds 7 and 8 suppressed the mRNA expression of CD147 in a dose-dependent manner, with an IC50 value of 3.38 ± 0.27 μM and 8.25 ± 0.33 μM, respectively. Besides, 7 dose-related down-regulated the mRNA expression of MMP-2, and MMP-9, having an IC50 value of 6.32 ± 0.31 μM and 11.57 ± 0.23 μM, respectively. Meanwhile, 8 at 10 μM reduced the mRNA expression of MMP-2 and MMP-9 by 35% and 25%, respectively. Significantly, the migration ability of the induced THP-1 cells was potently and dose-dependently inhibited by 7, with an IC50 value of 5.87 ± 0.27 μM.