A fully defined artificial diet for Myzus persicae – the detailed technical manual. Issue 6 (8th June 2020)

• Record Type: Journal Article
• Authors: van Emden, Helmut F.; Wild, Elizabeth A.
• Is Part Of: Entomologia experimentalis et applicata. Volume 168:Issue 6/7(2020)
• Page Start: 582
• Page End: 586
• Publication Date: 2020-06-08
• Subjects: aphid -- culturing -- laminar flow cabinet -- Parafilm -- phagostimulation -- sterilisation -- recipe -- Hemiptera -- Aphididae, artificial diet, Myzus persicae; Entomology -- Periodicals; 595.7
• DOI: 10.1111/eea.12917
• Languages: English
• ISSNs: 0013-8703

Abstract

Abstract: We have been able to maintain a continuous culture of Myzus persicae (Sulzer) (Hemiptera: Aphididae) on a fully defined artificial diet at Reading, UK, for over 30 years, without any return to plants. Following multiple requests from aphid researchers, here we provide the details of our culturing method. Detailed instructions are given for rearing M. persicae on the diet. An improved recipe is included as well as the construction and changing of diet sachets. Abstract : We have been able to maintain a continuous culture of Myzus persicae (Hemiptera: Aphididae) on a fully defined artificial diet at Reading, UK, for over 30 years, without any return to plants. Following multiple requests from aphid researchers, here we provide the details of our culturing method. Detailed instructions are given for rearing M. persicae on the diet. An improved recipe is included as well as the construction and changing of diet sachets.