Effects of an ophthalmic formulation of meloxicam on COX-2 expression, PGE2 release, and cytokine expression in a model of acute ocular inflammation. Issue 1 (25th October 2007)

• Record Type: Journal Article
• Authors: Cruz, R; Quintana-Hau, J D; González, J R; Tornero-Montaño, R; Baiza-Durán, L M; Vega, L
• Is Part Of: British journal of ophthalmology. Volume 92:Issue 1(2008)
• Page Start: 120
• Page End: 125
• Publication Date: 2007-10-25
• Subjects: Ophthalmology -- Periodicals; 617.7
• DOI: 10.1136/bjo.2007.125179
• Languages: English
• ISSNs: 0007-1161

Abstract

Abstract : Aim: To determine the efficacy of meloxicam ophthalmic formulation on COX-2 activity and expression, inflammation-related cytokines expression and inflammation in an ocular inflammation model. Methods: Ocular inflammation was induced in New Zealand rabbits by topical application of croton oil (3%) for 3 h. An ophthalmic solution of 0.03% meloxicam, 0.1% sodium diclofenac or vehicle (Sophisen™) was administered every 4 h. Conjunctiva, cornea, aqueous humour and vitreous humour were collected. Results: In irritated eyes, 72 h of meloxicam treatment downregulated COX-2 expression and activity (mRNA by RT-PCR and PGE2 levels by ELISA, respectively) in a time-dependent manner and reduced inflammation. Meanwhile, diclofenac failed to reduce COX-2 mRNA or PGE2 to basal levels after 7 days of treatment. Meloxicam treatment downregulated IL-6 and IFN-γ expression in the conjunctiva and IL-1β and TNF-α expression in the cornea. Diclofenac failed to modify these cytokines in both tissues. Meloxicam treatment increased the expression of IL-6 in conjunctiva, and IL-10 in cornea, while diclofenac had no effect on these cytokines. Conclusion: Meloxicam treatment was more efficient than diclofenac in downregulating the expression and activity of COX-2, reducing inflammation, and modifying the inflammatory-related cytokines.