Prauserella cavernicola sp. nov., isolated from cave rock. Issue 11 (22nd November 2021)
- Record Type:
- Journal Article
- Title:
- Prauserella cavernicola sp. nov., isolated from cave rock. Issue 11 (22nd November 2021)
- Main Title:
- Prauserella cavernicola sp. nov., isolated from cave rock
- Authors:
- Suriyachadkun, Chanwit
Ngaemthao, Wipaporn
Pujchakarn, Tawanmol
Mhuantong, Wuttichai
Chunhametha, Suwanee - Abstract:
- Abstract : A novel actinomycete, designated strain ASG 168 T, was isolated from cave rock collected from Stegodon Sea Cave in Thailand. Long chains of non-motile spores that were oval or spherical in shape with a smooth surface developed on aerial mycelia. Substrate mycelia fragmented into irregular rod-shaped elements. A polyphasic taxonomic study showed that strain ASG 168 T had typical characteristics of members of the genus Prauserella . 16S rRNA gene sequence analysis indicated that strain ASG 168 T shared 97.5 % similarity with Prauserella marina MS498 T and 96.7 % with Prauserella coralliicola SCSIO 11529 T . Average nucleotide identity values with P. coralliicola SCSIO 11529 T and P. marina MS498 T were 82.98 and 76.08 %, respectively. The cell-wall peptidoglycan contained meso -diaminopimelic acid. The whole-cell sugars contained ribose, arabinose and galactose. The predominant menaquinone was MK-9(H4 ). The predominant fatty acids were iso-C16 : 0, C16 : 0 and summed feature 3 (C16 : 1 ω 7 c /C16 : 1 ω 6 c ). The phospholipid profile consisted of phosphatidylethanolamine, phosphatidylmethylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides and two unknown phospholipids. The G+C content of the genomic DNA was 70.6 mol%. Differentiation of strain ASG 168 T from closely related species was evident from digital DNA–DNA hybridization values of 29.2 and 21.3 % with P. coralliicola and P. marina, respectively.Abstract : A novel actinomycete, designated strain ASG 168 T, was isolated from cave rock collected from Stegodon Sea Cave in Thailand. Long chains of non-motile spores that were oval or spherical in shape with a smooth surface developed on aerial mycelia. Substrate mycelia fragmented into irregular rod-shaped elements. A polyphasic taxonomic study showed that strain ASG 168 T had typical characteristics of members of the genus Prauserella . 16S rRNA gene sequence analysis indicated that strain ASG 168 T shared 97.5 % similarity with Prauserella marina MS498 T and 96.7 % with Prauserella coralliicola SCSIO 11529 T . Average nucleotide identity values with P. coralliicola SCSIO 11529 T and P. marina MS498 T were 82.98 and 76.08 %, respectively. The cell-wall peptidoglycan contained meso -diaminopimelic acid. The whole-cell sugars contained ribose, arabinose and galactose. The predominant menaquinone was MK-9(H4 ). The predominant fatty acids were iso-C16 : 0, C16 : 0 and summed feature 3 (C16 : 1 ω 7 c /C16 : 1 ω 6 c ). The phospholipid profile consisted of phosphatidylethanolamine, phosphatidylmethylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides and two unknown phospholipids. The G+C content of the genomic DNA was 70.6 mol%. Differentiation of strain ASG 168 T from closely related species was evident from digital DNA–DNA hybridization values of 29.2 and 21.3 % with P. coralliicola and P. marina, respectively. Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel actinomycete strain ASG 168 T (=TBRC 13679 T =NBRC 114887 T ) is proposed to be the type strain of a novel species, Prauserella cavernicola sp. nov. … (more)
- Is Part Of:
- International journal of systematic and evolutionary microbiology. Volume 71:Issue 11(2021)
- Journal:
- International journal of systematic and evolutionary microbiology
- Issue:
- Volume 71:Issue 11(2021)
- Issue Display:
- Volume 71, Issue 11 (2021)
- Year:
- 2021
- Volume:
- 71
- Issue:
- 11
- Issue Sort Value:
- 2021-0071-0011-0000
- Page Start:
- Page End:
- Publication Date:
- 2021-11-22
- Subjects:
- cave -- Prauserella cavernicola -- rock -- Thailand
Microbiology -- Periodicals
Microbiology -- Classification -- Periodicals
579 - Journal URLs:
- https://www.microbiologyresearch.org/content/journal/ijsem ↗
- DOI:
- 10.1099/ijsem.0.005107 ↗
- Languages:
- English
- ISSNs:
- 1466-5026
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library HMNTS - ELD Digital store
- Ingest File:
- 19845.xml