Anti‐biofilm potential of kefir‐derived Lactobacillus paracasei L10 against Vibrio parahaemolyticus. (10th October 2021)

• Record Type: Journal Article
• Authors: Shangguan, W.; Xie, T.; Zhang, R.; Lu, C.; Han, X.; Zhong, Q.
• Is Part Of: Letters in applied microbiology. Volume 73:Number 6(2021)
• Page Start: 750
• Page End: 758
• Publication Date: 2021-10-10
• Subjects: anti‐biofilm -- kefir -- Lactobacillus paracasei L10 -- microscopic visualization -- Vibrio parahaemolyticus; Microbiology -- Periodicals; 660.62
• DOI: 10.1111/lam.13568
• Languages: English
• ISSNs: 0266-8254

Abstract

Abstract: Vibrio parahaemolyticus, a kind of biofilm‐forming foodborne bacterium, presents formidable challenges to the effectiveness of antimicrobial agents. Increasingly, the safety of chemical antimicrobials has aroused the widespread attention of the public. The development of the novel nature antimicrobial agents has become critical for controlling biofilm‐related pollution and infections. In this paper, we investigated the antibacterial activity of Lactobacillus paracasei L10, and evaluated the inhibition and eradication effects of the cell‐free supernatant (CFS) of the strain on V. parahaemolyticus biofilms in detail. We found that the CFS exhibited marked antibacterial activity against all tested pathogenic bacteria. In co‐cultural assay, L. paracasei L10 could notably reduce cell viability in both plankton and biofilm of V. parahaemolyticus and this antagonism effect in biofilm was greater than that in planktonic state. Meanwhile, the growth of V. parahaemolyticus was completely inhibited when 6% (v/v) of the CFS was added, and the supernatant also showed a concentration‐dependent manner to inhibit and eradicate the biofilms of V. parahaemolyticus while decreased the metabolic activity of the biofilm in the same way. Moreover, the fluorescence microscopic and confocal laser scanning microscopy images confirmed the anti‐biofilm activity of the CFS. This study elucidates that L. paracasei L10 displays a significant anti‐biofilm effect on V. parahaemolyticus and the mechanism of its antagonism merits further study, which provides theoretical support for further development and application of L. paracasei L10 as anti‐biofilm agents. Abstract : Significance and Impact of the Study: The potential harm of Vibrio parahaemolyticus existing in biofilm on public health cannot be neglected. It is of great significance to apply lactic acid bacteria (LAB) as a safe biological strategy to control the pathogenicity and drug resistance of pathogens enhanced by biofilms. This work mainly evaluated the anti‐biofilm effects of one strain of kefir‐derived LAB on V. parahaemolyticus . The results exhibited that Lactobacillus paracasei L10 could effectively inhibit biofilm formation and eradicate established biofilms of V. parahaemolyticus . Our findings support that L. paracasei L10 has the potential to be applied as a natural and effective anti‐biofilm agent to control the contamination and infection of V. parahaemolyticus .