Bioengineering Approaches for the Advanced Organoid Research. Issue 45 (24th September 2021)

• Record Type: Journal Article
• Authors: Yi, Sang Ah; Zhang, Yixiao; Rathnam, Christopher; Pongkulapa, Thanapat; Lee, Ki‐Bum
• Is Part Of: Advanced materials. Volume 33:Issue 45(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-09-24
• Subjects: bioengineered organoids -- biomaterials -- disease modeling -- extracellular matrix -- genetic engineering; Materials -- Periodicals; Chemical vapor deposition -- Periodicals; 620.11
• DOI: 10.1002/adma.202007949
• Languages: English
• ISSNs: 0935-9648

Abstract

Abstract: Recent advances in 3D cell culture technology have enabled scientists to generate stem cell derived organoids that recapitulate the structural and functional characteristics of native organs. Current organoid technologies have been striding toward identifying the essential factors for controlling the processes involved in organoid development, including physical cues and biochemical signaling. There is a growing demand for engineering dynamic niches characterized by conditions that resemble in vivo organogenesis to generate reproducible and reliable organoids for various applications. Innovative biomaterial‐based and advanced engineering‐based approaches have been incorporated into conventional organoid culture methods to facilitate the development of organoid research. The recent advances in organoid engineering, including extracellular matrices and genetic modulation, are comprehensively summarized to pinpoint the parameters critical for organ‐specific patterning. Moreover, perspective trends in developing tunable organoids in response to exogenous and endogenous cues are discussed for next‐generation developmental studies, disease modeling, and therapeutics. Abstract : The emergence of organoid technologies, in terms of development of 3D miniature organs in vitro, has provided revolutionized approaches for understanding developmental processes and disease modeling. Comprehensive insights into the materials and genome engineering techniques applied to organoid culture are demonstrated. Based on the recent achievements, prospects and key challenges for the next‐generation organoid technologies are also discussed.