Optimization, extraction, and purification of three bioactive compounds from Entada phaseoloides by high‐speed countercurrent chromatography. (6th September 2021)

• Record Type: Journal Article
• Authors: Zhou, Yan; Shan, Hu; Lü, Haitao
• Is Part Of: Biomedical chromatography. Volume 35:Number 12(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-09-06
• Subjects: antioxidant activity, Entada phaseoloides, entadamide A, entadamide A‐β‐D‐glucopyranoside, high‐speed countercurrent chromatography, phaseoloidin; Chromatographic analysis -- Periodicals; Biology -- Periodicals; Medicine -- Periodicals; Biology -- Periodicals; Chromatography -- methods -- Periodicals; Medicine -- Periodicals; 543.089
• DOI: 10.1002/bmc.5232
• Languages: English
• ISSNs: 0269-3879

Abstract

Abstract: The objective of this paper was to develop a preparative method for the separation and purification of phaseoloidin, entadamide A, and entadamide A‐ β ‐D ‐glucopyranoside from the crude extract of Entada phaseoloides by high‐speed countercurrent chromatography (HSCCC) for the first time. Optimized by orthogonal experiments, the extraction conditions were extraction temperature of 65°C, solid‐to‐liquid ratio of 1:15 (g/mL), ethanol concentration of 40%, and extraction time of 2.5 h. Using n ‐butanol‐acetic acid‐water (4:1:5, v/v/v) as the two‐phase solvent system, 38.79 mg phaseoloidin (the purity was 99.3% with a recovery of 98.1%), 34.85 mg entadamide A (the purity was 96.4% with a recovery of 98.5%), and 33.97 mg entadamide A‐ β ‐D ‐glucopyranoside (the purity was 98.6% with a recovery of 97.7%) were obtained from 500 mg crude extract by HSCCC in head‐to‐tail elution mode. The retention ratio of stationary phase was 51.0%. According to the antioxidant activity assays, phaseoloidin, entadamide A, and entadamide A‐ β ‐D ‐glucopyranoside had certain scavenging abilities on 1, 1‐diphenyl‐2‐picrylhydrazyl free radicals and hydroxyl free radicals.