Electrochemical Activity Produced from Abundant Expression of C‐Type Cytochromes in a Filamentous Gram‐Positive Bacterium. Issue 21 (5th November 2021)

• Record Type: Journal Article
• Authors: Li, Daobo; Zheng, Xiaodan; Yang, Yonggang; Kong, Guannan; Xu, Meiying
• Is Part Of: ChemElectroChem. Volume 8:Issue 21(2021)
• Page Start: 4124
• Page End: 4128
• Publication Date: 2021-11-05
• Subjects: Spectroelectrochemistry -- Electron transport -- c-type cytochrome -- Redox potential -- Bacteria; Electrochemistry -- Periodicals; 541.37
• DOI: 10.1002/celc.202101023
• Languages: English
• ISSNs: 2196-0216

Abstract

Abstract: Natural microbes employing c ‐type cytochromes ( c ‐Cyts) for extracellular electron transport (EET) were greatly valuable to develop redox‐based bioelectrochemical applications. However, low levels of c ‐Cyt expression limited the phylogenetically diverse Gram‐positive species to be used in bioelectrochemical devices. This work reported the remarkable finding that a high level of c ‐Cyts was expressed in the cells of the Gram‐positive strain Lysinibacillus varians GY32. c ‐Cyts in Lysinibacillus varians GY32 cells were expressed at a level close to those in Shewanella oneidensis . These c ‐Cyts were found to cluster in cytoplasmic membrane and periplasmic space, along the length of Lysinibacillus varians GY32 cell. With voltammetry and spectroelectrochemical titration, phenazine methosulfate‐mediated electron transfer between the c ‐Cyts and an electrode was proved. These results expanded the accessible natural models of EET and suggested a new microbial platform for developing bioelectrochemical applications. Abstract : Bioelectrochemical insider : A filamentous Gram‐positive bacterium Lysinibacillus varians GY32 was uncovered to express a high level of c ‐type cytochromes, forming a redox‐active structure sheathed in the mediator‐permeable cell wall and thus providing a unique model to develop bioelectrochemical applications.