Fabrication of nano-decorated ZnO-fibrillar chitosan exhibiting a superior performance as a promising replacement for conventional ZnO. (15th November 2021)

• Record Type: Journal Article
• Authors: Zabihi, Erfan; Arab-Bafrani, Zahra; Hoseini, Seyyed Morteza; Mousavi, Elham; Babaei, Amir; Khalili, Mohsen; Hashemi, Mohammad Mostakhdem; Javid, Naeme
• Is Part Of: Carbohydrate polymers. Volume 274(2021)
• Publication Date: 2021-11-15
• Subjects: Zebrafish -- Nanotoxicity -- Bio-nanohybrid -- Antibacterial activity -- Nano-fibrillar chitosan; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2021.118639
• Languages: English
• ISSNs: 0144-8617

Abstract

Abstract: In this research, bioactive nano-hybrids based on the nano-fibrillar chitosan-ZnO (NF-CS-ZnO) were synthesized to diminish the toxicity of ZnO-NPs. The successful formation of nano-hybrids was confirmed by FT-IR, UV–Vis, and FE-SEM analyses, showing a uniform spherical ZnO-NPs with an average diameter of 20–30 nm, homogeneously dispersed on NF-CS. The obtained results demonstrated a remarkable antibacterial activity of NF-CS-ZnO-0.6 nano-hybrid against E. coli and S. aureus and, interestingly, no cytotoxic on normal cells (even at a high concentration of 100 μg/mL). Furthermore, NF-CS hybridization efficiently decreased the up-regulation in Cas3, Cas9, and Il6 of inspected fishes compared to the ZnO-NPs. Histopathological examination revealed hepatocyte necrosis in the fish exposed to ZnO-NPs and hyperemia exposed to NF-CS-ZnO-0.6 nano-hybrid. Finally, NF-CS efficiently improved the bio-safety and bactericidal activity of ZnO-NPs; therefore, NF-CS-ZnO nano-hybrid is prominently recommended as a talented low-toxicity antibacterial agent replacement of conventional ZnO-NPs for use in different applications. Graphical abstract: Unlabelled Image Highlights: NF-CS-ZnO nano-hybrids were successfully fabricated by the co-precipitation method. NF-CS hybridization efficiently improved the biological performance of ZnO-NPs. Zinc ion release of NF-CS-ZnO nano-hybrids were lower than that of ZnO-NPs. NF-CS-ZnO nano-hybrids decreased in-vivo inflammation, apoptosis and hepatic damages.