Classification and coding of platelet-rich plasma derived from New Zealand white rabbits for tissue engineering and regenerative medicine applications. (2nd November 2021)

• Record Type: Journal Article
• Authors: Sharun, Khan; Pawde, Abhijit M.; Manjusha, K. M.; Banu S, Amitha; Kalaiselvan, E.; Kumar, Rohit; Kinjavdekar, Prakash; Amarpal, ; Verma, Med Ram
• Is Part Of: Expert opinion on biological therapy. Volume 21:Number 11(2021)
• Page Start: 1473
• Page End: 1482
• Publication Date: 2021-11-02
• Subjects: Platelet-rich plasma -- New Zealand white rabbits -- standardization -- characterization -- classification; Gene therapy -- Periodicals; Protein drugs -- Periodicals; Peptide drugs -- Periodicals; Immunotherapy -- Periodicals; Drug delivery systems -- Periodicals; 615.5
• DOI: 10.1080/14712598.2021.1955099
• Languages: English
• ISSNs: 1471-2598

Abstract

ABSTRACT: Background and objective: Platelet-rich plasma (PRP) is a category of platelet concentrate that has been widely used as a therapeutic modality in musculoskeletal medicine. The present study was conducted to classify and code the non-activated platelet-rich plasma (nPRP) derived from New Zealand white rabbits for tissue engineering and other regenerative medicine applications. Methods: PRP was prepared from the whole blood by double centrifugation protocol using a laboratory centrifuge. The prepared nPRP was characterized using the parameters such as platelet dose, the relative composition of platelets, WBC, and RBC. The production protocol was described using the parameters such as platelet enrichment factor, factor increase in WBC concentration, platelet capture efficiency, WBC-reducing efficiency, and RBC-reducing efficiency. The nPRP was also classified and coded using the most recent and universally accepted classification and coding systems. Results: The non-activated leukocyte-poor red cell-rich PRP had an average platelet count of 1875.75 × 10 9 /L, which is higher than the basal platelet concentration in the whole blood. Furthermore, the protocol used in this study has a mean platelet capture efficiency of 47.43 ± 6.42%. Conclusion: The protocol described in this study can be used to prepare non-activated leukocyte-poor red cell-rich PRP (Red-PRP IC1) from rabbits that can be coded as 318–00-00.