Strategies for organelle targeting of fluorescent probes. Issue 43 (13th September 2021)

• Record Type: Journal Article
• Authors: Lin, Jiarun; Yang, Kylie; New, Elizabeth J.
• Is Part Of: Organic & biomolecular chemistry. Volume 19:Issue 43(2021)
• Page Start: 9339
• Page End: 9357
• Publication Date: 2021-09-13
• Subjects: Chemistry, Organic -- Periodicals; Bioorganic chemistry -- Periodicals; Chemistry, Physical organic -- Periodicals; 547
• DOI: 10.1039/d1ob01447a
• Languages: English
• ISSNs: 1477-0520

Abstract

Abstract : Here we provide a comprehensive review of methods for targeting fluorescent cargo to sub-cellular organelles. Abstract : Fluorescent tools have emerged as an important tool for studying the distinct chemical microenvironments of organelles, due to their high specificity and ability to be used in non-destructive, live cellular studies. These tools fall largely in two categories: exogenous fluorescent dyes, or endogenous labels such as genetically encoded fluorescent proteins. In both cases, the probe must be targeted to the organelle of interest. To date, many organelle-targeted fluorescent tools have been reported and used to uncover new information about processes that underpin health and disease. However, the majority of these tools only apply a handful of targeting groups, and less-studied organelles have few robust targeting strategies. While the development of new, robust strategies is difficult, it is essential to develop such strategies to allow for the development of new tools and broadening the effective study of organelles. This review aims to provide a comprehensive overview of the major targeting strategies for both endogenous and exogenous fluorescent cargo, outlining the specific challenges for targeting each organelle type and as well as new developments in the field.