Brevibacillus composti sp. nov., isolated from hyperthermophilic compost. Issue 11 (11th November 2021)

• Record Type: Journal Article
• Authors: Tang, Rong; Zhang, Qi; Ye, Yin; Yang, Shang; Fu, Tao; Liu, Guo-Hong; Zhou, Shun-Gui
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 71:Issue 11(2021)
• Publication Date: 2021-11-11
• Subjects: Brevibacillus composti sp. nov. -- hyperthermophilic compost -- thermophile; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.005098
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : Two aerobic, Gram-stain-positive, rod-shaped, endospore-forming, thermophilic bacterial strains, designated FJAT-54423 T and FJAT-54424, were isolated from hyperthermophilic compost sampled in Shanxi Province, PR China. Growth was observed at 30–60 °C (optimum, 50 °C) and pH 6.0–9.0 (optimum, pH 7.0), with up to 2.0 % (w/v) NaCl (optimum, 0 % NaCl). The 16S rRNA gene sequence similarity between FJAT-54423 T and FJAT-54424 was 99.9%, and the maximum similarity to a valid taxon was observed with Brevibacillus borstelensis (98.3%). Further, in phylogenetic and phylogenomic trees, strains FJAT-54423 T and FJAT-54424 branched with members of the genus Brevibacillus . The menaquinone was MK-7, and the major fatty acids were iso-C15 : 0 and anteiso-C15 : 0 . The main polar lipids included phosphatidylmethylethanolamine, phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The cell-wall peptidoglycan was found to contain meso -diaminopimelic acid. The DNA G+C content of strains FJAT-54423 T and FJAT-54424 were 54.3 and 54.4 mol%, respectively. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values of strain FJAT-54423 T and its most closely related reference strain B. borstelensis DSM 6347 T were 77.7 and 21.5 %, respectively, which were lower than the recommended species delineation thresholds of ANI (95%) and dDDH (70%). Based on the observed physiological properties, chemotaxonomic characteristics and ANI and dDDH values, FJAT-54423 T and FJAT-54424 belong to a novel species of the genus Brevibacillus, for which the name Brevibacillus composti sp. nov. is proposed. The type strain is FJAT-54423 T (=GDMCC 1.2054 T =KCTC 43273 T ).