Comammox activity dominates nitrification process in the sediments of plateau wetland. (1st November 2021)

• Record Type: Journal Article
• Authors: Yuan, Dongdan; Zheng, Lei; Tan, Qiuyang; Wang, Xue; Xing, Yuzi; Wang, Huipeng; Wang, Shanyun; Zhu, Guibing
• Is Part Of: Water research. Volume 206(2021)
• Publication Date: 2021-11-01
• Subjects: Plateau wetland sediments -- Elevation ranges -- Comammox activity -- Key comammox species -- Biogeochemical driving pattern; Water -- Pollution -- Research -- Periodicals; 363.7394
• DOI: 10.1016/j.watres.2021.117774
• Languages: English
• ISSNs: 0043-1354

Abstract

Highlights: Comammox bacteria were widely distributed in plateau wetland sediments. Comammox activity dominates nitrification process in plateau wetland sediments. Elevation always had the strongest effects on the key comammox species. Elevation may drive niche separation of key comammox species. The niche separation of key comammox species may lead to high contribution. Abstract: The recent discovery of complete ammonia oxidation (comammox) has increased our understanding of nitrification. Although comammox has been shown to play an important role in plain wetland ecosystems, studies of comammox contribution are still limited in plateau wetland ecosystems. Here, we analyzed the abundance, activity, community and biogeochemical mechanisms of the comammox bacteria in Yunnan-kweichow and Qinghai-Tibet plateau wetlands from elevations of 1000-5000 m. Comammox bacteria were widely distributed in all 16 sediment samples with abundances higher than 0.96 ± 0.26 × 10 7 copies g −1 ( n = 16). Comammox showed high activity (1.18 ± 0.17 to 1.98 ± 0.08 mg N kg −1 d −1 ) at high-elevation (3000-5000 m) and dominated the nitrification process (activity contribution: 37.20 - 60.62%). The activity contribution of ammonia-oxidizing bacteria (1.07 ± 0.08 to 2.79 ± 0.35 mg N kg −1 d −1 ) dominated the nitrification process (44.55 - 64.15%) in low-elevation (1000-3000 m) samples. All detected comammox Nitrospira belonged to clade A, while clade B was not detected. Elevation always had a strongest effect on key comammox species. Thus, we infer that elevation may drive the high relative abundance of the species Candidatus Nitrospira nitrificans (avg. 12.40%) and the low relative abundance of the species Nitrospira sp. SG-bin2 (avg. 4.75%) in high-elevation samples that showed a high comammox activity (avg. 1.62 mg N kg −1 d −1 ) and high contribution (avg. 46.08%) to the nitrification process. These results indicate that comammox may be an important and currently underestimated microbial nitrification process in plateau wetland ecosystems. Graphical abstract: Image, graphical abstract