Transcriptome-wide study in the green microalga Messastrum gracile SE-MC4 identifies prominent roles of photosynthetic integral membrane protein genes during exponential growth stage. (December 2021)

• Record Type: Journal Article
• Authors: Wan Afifudeen, C.L.; Aziz, Ahmad; Wong, Li Lian; Takahashi, Kazutaka; Toda, Tatsuki; Abd Wahid, Mohd Effendy; Cha, Thye San
• Is Part Of: Phytochemistry. Volume 192(2021)
• Publication Date: 2021-12
• Subjects: Messastrum gracile, chlorophyta -- Selenastraceae -- de novo transcriptome -- Biomass enhancement; Botanical chemistry -- Periodicals; Biochemistry -- Periodicals; Botany -- Periodicals; Chimie végétale -- Périodiques; 572.2
• DOI: 10.1016/j.phytochem.2021.112936
• Languages: English
• ISSNs: 0031-9422

Abstract

Abstract: The non-model microalga Messastrum gracile SE-MC4 is a potential species for biodiesel production. However, low biomass productivity hinders it from passing the life cycle assessment for biodiesel production. Therefore, the current study was aimed at uncovering the differences in the transcriptome profiles of the microalgae at early exponential and early stationary growth phases and dissecting the roles of specific differential expressed genes (DEGs) involved in cell division during M. gracile cultivation. The transcriptome analysis revealed that the photosynthetic integral membrane protein genes such as photosynthetic antenna protein were severely down-regulated during the stationary growth phase. In addition, the signaling pathways involving transcription, glyoxylate metabolism and carbon metabolism were also down-regulated during stationary growth phase. Current findings suggested that the coordination between photosynthetic integral membrane protein genes, signaling through transcription and carbon metabolism classified as prominent strategies during exponential growth stage. These findings can be applied in genetic improvement of M. gracile for biodiesel application. Graphical abstract: Image 1 Highlights: Transcriptome between growth stages was analyzed to measure DEG during exponential growth stage. Rapid cell proliferation in M. gracile depends on optimum photosynthesis performance. Reduction in gene expression of IMP affects photosynthesis which leading to reduction in growth. Down-regulation of transcription genes during stationary growth hinder cell proliferation. Reduction in glyoxylate and carbon metabolism genes suggest limitation towards growth process.