Inside a Shell—Organometallic Catalysis Inside Encapsulin Nanoreactors. (1st October 2021)

• Record Type: Journal Article
• Authors: Lohner, Philipp; Zmyslia, Mariia; Thurn, Johann; Pape, Jasmin K.; Gerasimaitė, Rūta; Keller‐Findeisen, Jan; Groeer, Saskia; Deuringer, Benedikt; Süss, Regine; Walther, Andreas; Hell, Stefan W.; Lukinavičius, Gražvydas; Hugel, Thorsten; Jessen‐Trefzer, Claudia
• Is Part Of: Angewandte Chemie. Volume 133:Number 44(2021)
• Page Start: 24028
• Page End: 24034
• Publication Date: 2021-10-01
• Subjects: encapsulins -- homogeneous catalysis -- MINFLUX -- self-assembly -- single-molecule FRET; Chemistry -- Periodicals; 540
• DOI: 10.1002/ange.202110327
• Languages: English
• ISSNs: 0044-8249

Abstract

Abstract: Compartmentalization of chemical reactions inside cells are a fundamental requirement for life. Encapsulins are self‐assembling protein‐based nanocompartments from the prokaryotic repertoire that present a highly attractive platform for intracellular compartmentalization of chemical reactions by design. Using single‐molecule Förster resonance energy transfer and 3D‐MINFLUX analysis, we analyze fluorescently labeled encapsulins on a single‐molecule basis. Furthermore, by equipping these capsules with a synthetic ruthenium catalyst via covalent attachment to a non‐native host protein, we are able to perform in vitro catalysis and go on to show that engineered encapsulins can be used as hosts for transition metal catalysis inside living cells in confined space. Abstract : A bioorthogonal catalysis strategy has been developed inside a confined space using encapsulins equipped with HaloTag and [CpRu(hydroxyquinoline)(allyl)]PF6 . The strategy opens up a toolbox for designing compartmentalized „new‐to‐biology" reactions inside living cells.