Detection of immunoglobulin heavy chain gene rearrangements in Hodgkin's disease using PCR. Issue 10 (October 1993)

• Record Type: Journal Article
• Authors: Angel, C A; Pringle, J H; Primrose, L; Lauder, I
• Is Part Of: Journal of clinical pathology. Volume 46:Issue 10(1993)
• Page Start: 940
• Page End: 942
• Publication Date: 1993-10
• Subjects: Pathology -- Periodicals; Pathology, Molecular -- Periodicals; 616.0705
• DOI: 10.1136/jcp.46.10.940
• Languages: English
• ISSNs: 0021-9746

Abstract

Abstract : AIM--To detect clonal rearrangements of the immunoglobulin (Ig) heavy chain gene in Hodgkin's disease tissue using the polymerase chain reaction (PCR). METHODS--DNA extracted from 36 samples of Hodgkin's disease was analysed using PCR and primers from conserved sequences in the variable (VH) and joining (JH) regions. RESULTS--Clonal rearrangement was detected only in one case. Evidence of clonal immunoglobulin gene rearrangement had been detected previously in this case using conventional Southern blot analysis. CONCLUSIONS--The sensitivity of the two techniques is equivalent and clonal Ig heavy chain gene rearrangements are rare in Hodgkin's disease.