Altered CD45 expression in C77G carriers influences immune function and outcome of hepatitis C infection. Issue 8 (27th February 2006)

• Record Type: Journal Article
• Authors: Dawes, R; Hennig, B; Irving, W; Petrova, S; Boxall, S; Ward, V; Wallace, D; Macallan, D C; Thursz, M; Hill, A; Bodmer, W; Beverley, P C L; Tchilian, E Z
• Is Part Of: Journal of medical genetics. Volume 43:Issue 8(2006)
• Page Start: 678
• Page End: 684
• Publication Date: 2006-02-27
• Subjects: HCV, hepatitis C virus -- HENCORE, Hepatitis C European Network for Cooperative Research -- Lck, lymphocyte specific protein tyrosine kinase -- PBMC, peripheral blood mononuclear cells -- SNP, single nucleotide polymorphism -- TcR, T cell receptor; CD45 -- C77G variant -- hepatitis C -- immune response; Medical genetics -- Periodicals; 616.042
• DOI: 10.1136/jmg.2005.040485
• Languages: English
• ISSNs: 1468-6244

Abstract

Abstract : Background: A polymorphism in exon 4 (C77G) of CD45 that alters CD45 splicing has been associated with autoimmune and infectious diseases in humans. Objective: To investigate the effect of C77G in hepatitis C virus (HCV) infected individuals and study the phenotype and function of peripheral blood mononuclear cells (PBMC) from healthy and hepatitis C infected C77G carriers. Results: C77G individuals showed an increased proportion of primed CD45RA and effector memory CD8 T cells and more rapid activation of the lymphocyte specific protein tyrosine kinase (Lck) following CD3 stimulation. Transgenic mice with CD45 expression mimicking that in human C77G variants had more activated/memory T cells, more rapid proliferative responses, and activation of Lck. Conclusions: Changes in CD45 isoform expression can alter immune function in human C77G variants and CD45 transgenic mice. The C77G allele may influence the outcome of HCV infection.