Determination of Integrated HPV58 Sequences in Cervical Lesions. Issue 7 (1st September 2012)

• Record Type: Journal Article
• Authors: Li, Hui; Zhang, Ruifen; Cai, Yupin; Li, Yuan; Cheng, Xuemei; Zhu, Baoli; Yang, Yi; Xiang, Yang
• Is Part Of: International journal of gynecological cancer. Volume 22:Issue 7(2012)
• Page Start: 1234
• Page End: 1237
• Publication Date: 2012-09-01
• Subjects: Human papillomavirus 58 -- Integration -- Repetitive element -- Disruption of E1 gene; Generative organs, Female -- Cancer -- Periodicals; 616.99465
• DOI: 10.1097/IGC.0b013e31825d4716
• Languages: English
• ISSNs: 1048-891X

Abstract

Abstract : Integration of high-risk HPV in host genome is an important event in cervical cancer development. This study was aimed to analyze the integration of HPV58, a high-risk type that is prevalent in cervical lesions from southeastern Asia. Detection of integrated papillomavirus sequences by ligation-mediated polymerase chain reaction followed by DNA sequencing revealed 8 integrations in 5 samples, and virus integration was found present in 2 samples with early lesion. Sequence analysis of the viral-cellular junctions showed that E1 disruption in virus genome was an early and common event during HPV58 infection. In 6 integrations, DNA fragments of HPV58 genome integrated into the repetitive element sequences of host genome.