Two-photon ESIPT-based fluorescent probe using 4-hydroxyisoindoline-1, 3-dione for the detection of peroxynitrite. Issue 84 (7th October 2021)

• Record Type: Journal Article
• Authors: Wu, Luling; Tian, Xue; Lee, Dong Joon; Yoon, Juyoung; Lim, Chang Su; Kim, Hwan Myung; James, Tony D.
• Is Part Of: Chemical communications. Volume 57:Issue 84(2021)
• Page Start: 11084
• Page End: 11087
• Publication Date: 2021-10-07
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/d1cc03160k
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : Excited-state intramolecular proton transfer (ESIPT)-based fluorophores with two-photon excitation fluorescence (TPEF) are rare. Abstract : Excited-state intramolecular proton transfer (ESIPT)-based fluorophores with two-photon excitation fluorescence (TPEF) are rare. Our aim with this research was to develop ESIPT-based fluorophores exhibiting TPEF. Herein, we used 4-hydroxyisoindoline-1, 3-dione as a scaffold to develop a two-photon fluorescent probe BHID-Bpin, for the detection of peroxynitrite (ONOO − ). BHID-Bpin exhibits excellent selectivity, sensitivity, and fast response towards ONOO − in PBS buffer solution (10 mM, pH = 7.40). Additionally, BHID-Bpin displays high photo-stability under two-photon irradiation at 750 nm. Furthermore, the probe can image endogenous ONOO − in HeLa cells and exogenous ONOO − in rat hippocampal slices at a depth of 110 μm.