Ionozyme: ionic liquids as solvent and stabilizer for efficient bioactivation of CO2. Issue 18 (23rd August 2021)

• Record Type: Journal Article
• Authors: Ji, Xiuling; Xue, Yaju; Li, Zhuang; Liu, Yanrong; Liu, Lei; Busk, Peter Kamp; Lange, Lene; Huang, Yuhong; Zhang, Suojiang
• Is Part Of: Green chemistry. Volume 23:Issue 18(2021)
• Page Start: 6990
• Page End: 7000
• Publication Date: 2021-08-23
• Subjects: Environmental chemistry -- Industrial applications -- Periodicals; Environmental management -- Periodicals; 660
• DOI: 10.1039/d1gc02503a
• Languages: English
• ISSNs: 1463-9262

Abstract

Abstract : The first developed ionozyme that combines a discovered novel FDHPa with a synergistic ionic microenvironment enables efficient bioactivation of CO2 . Abstract : The efficient bioactivation of CO2 provides an alternative new strategy for producing high value chemicals and fuels. Normally, in vitro activation of CO2 can be achieved by using formate dehydrogenase (FDH). However, the CO2 solubility and the activity of commercial FDHCb remain a tough challenge for the efficient bioactivation of CO2 . Here, we report a new "ionozyme" strategy created by using ionic liquids (ILs) as a solvent and enzyme stabilizer, resulting in a 142.3-fold increase in CO2 conversion over FDHCb. The ionozyme (FDHPa-[CH][Pro]-[TMGH][PhO]) was first developed by combing a discovered novel FDHPa with an efficient synergistic ionic microenvironment. The remarkable performance of this ionozyme is attributed to forming key intermediates [CH][ProCOO] and [TMGH][PhOCOO], stabilizing the enzyme structure with increased solvation structure, and shortening the distance (3.9 Å) between NADH and CO2 to favor the hydride transfer by facilitating their relative orientation and forming new hydrogen bonds at the active sites. This bioactivation of CO2 by this specific ionozyme represents ideal starting points for the sustainable carbon cycle.