Flexible electrochemical aptasensor for cortisol detection in human sweat. Issue 36 (24th August 2021)

• Record Type: Journal Article
• Authors: Mugo, Samuel M.; Alberkant, Jonathan; Bernstein, Nina; Zenkina, Olena V.
• Is Part Of: Analytical methods. Volume 13:Issue 36(2021)
• Page Start: 4169
• Page End: 4173
• Publication Date: 2021-08-24
• Subjects: Chemistry, Analytic -- Periodicals; Analytical biochemistry -- Periodicals; Chemical laboratories -- Standards -- Periodicals; 543.1905
• DOI: 10.1039/d1ay01233a
• Languages: English
• ISSNs: 1759-9660

Abstract

Abstract : An inexpensive flexible PDMS@CNC/CNT@aptamer sensor for facile detection of cortisol in human sweat with capacity for use as wearable device. Abstract : This communication demonstrates an electrochemical DNA aptasensor for the detection of cortisol in human sweat. The aptasensor was fabricated via layer-by-layer assembly on stretchable polydimethylsiloxane (PDMS) coated with conductive nanoporous carbon nanotube-cellulose nanocrystals (CNC/CNT) film using a linker to a cortisol specific DNA aptamer. The flexible cortisol aptasensor had a dynamic range of 2.5–35 ng mL −1 . The aptasensor precision was determined to be 2.7% relative standard deviation (%RSD) across the concentration dynamic range. The aptasensor was determined to have a limit of detection (LOD) of ∼ 1.8 ng mL −1 . The aptasensor was demonstrated to have high selectivity to cortisol and was unresponsive to interfering species including glucose, sodium lactate, and β-estradiol. The aptasensor was successfully evaluated for the detection of cortisol in human sweat indicative of its high specificity.