Application strategies of peptide nucleic acids toward electrochemical nucleic acid sensors. Issue 19 (13th August 2021)

• Record Type: Journal Article
• Authors: Lai, Qingteng; Chen, Wei; Zhang, Yanke; Liu, Zhengchun
• Is Part Of: Analyst. Volume 146:Issue 19(2021)
• Page Start: 5822
• Page End: 5835
• Publication Date: 2021-08-13
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/d1an00765c
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : Various construction protocols of PNAs-based electrochemical nucleic acid sensor enables the feasibility of rapid nucleic acid diagnosis in genetics, pathology, criminology, and food safety. Abstract : Peptide nucleic acids (PNAs) have attracted tremendous interest in the fabrication of highly sensitive electrochemical nucleic acid biosensors due to their higher stability and increased sensitivity than common DNA probes. The neutral pseudopeptide backbone of PNAs not only makes the PNA/DNA duplexes more stable but also provides many opportunities to construct ultrasensitive nucleic acid sensors. This review presents the details of various protocols for the construction of PNA-based electrochemical nucleic acid sensors. The crucial factors, origin, and development of PNA, immobilization methods of PNA probes and signal generation mechanisms, are discussed. This review aims to provide a reference for ultrasensitive PNA electrochemical biosensor preparation.