204 Performance and diagnostic accuracy of human papillomavirus testing on urine and self-collected vaginal samples in a referral population. (4th December 2020)

• Record Type: Journal Article
• Authors: Cho, Hyun-Woong; Lee, Jae Kwan; Hong, Jin Hwa
• Is Part Of: International journal of gynecological cancer. Volume 30(2020)Supplement 4
• Page Start: A7
• Page End: A7
• Publication Date: 2020-12-04
• Subjects: Generative organs, Female -- Cancer -- Periodicals; 616.99465
• DOI: 10.1136/ijgc-2020-ESGO.15
• Languages: English
• ISSNs: 1048-891X

Abstract

Abstract : Introduction/Background: Human papillomavirus (HPV) is well established as the main cause of cervical cancer. Non-invasive self-collected urine and vaginal sampling have the potential advantage of increasing patient compliance with cervical cancer screening. Methodology: Women referred for colposcopy at Korea University Guro Hospital, following abnormal cytology, were included this study. A total of 314 paired urine, vaginal and cervical samples were collected. Primary endpoints were sensitivity for CIN2+/CIN3+ and specificity for <CIN2; secondary endpoints were the relative accuracy of hrHPV test results in vaginal and urine samples versus cervical samples. Results: For clinician-collected cervical samples, Sejong Realtime HR-S HPV test sensitivity for detecting and specificity from were similar to well-established test (Anyplex™ II HPV 28) [sensitivity for CIN3+ (n=109) 93.27% (95% confidence interval (CI), 86.62–97.25); CIN2+ (n=130) 92.74% (95% CI, 86.67–96.63); specificity for<CIN2 31.82% (95% CI, 25.01–39.25)]. All the paired differences (cervical versus urine sampling, cervix versus vaginal sampling) in sensitivity were statistically significant. However, among women with ASCUS/LSIL cytology, hrHPV sensitivity on vaginal samples was comparable to that of cervical samples for detection of CIN2+ and CIN3+ lesions. In addition, hrHPV sensitivity of Anyplex II HPV 28 assay on urine was comparable to that of cervical samples for detection of CIN3+ lesions (p=0.07) in women with ASCUS/LSIL cytology. Conclusion: HPV tests using urine and vaginal samples were still inferior to clinician-collected cervical samples in terms of detecting CIN2/3. However, these results indicate that combination of cytology with reflex hrHPV test using vaginal and urine samples may offer a reliable strategy for discriminating women at greater risk of precancerous lesion, increasing compliance of patients. Disclosures: I have no conflict of interest to disclose.