A high sensitivity electrochemical sensor based on a dual-template molecularly imprinted polymer for simultaneous determination of clenbuterol hydrochloride and ractopamine. Issue 20 (23rd September 2021)

• Record Type: Journal Article
• Authors: Li, Xiang; Li, Yangguang; Yu, Pai; Tong, Yanbin; Ye, Bang-Ce
• Is Part Of: Analyst. Volume 146:Issue 20(2021)
• Page Start: 6323
• Page End: 6332
• Publication Date: 2021-09-23
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/d1an01413g
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : A molecularly imprinted polymer grown on a nitrogen-doped Fe-MOF modified glassy carbon electrode for the detection of clenbuterol hydrochloride and ractopamine in humans. Abstract : A nitrogen-doped Fe-MOF shows a high specific surface area and excellent electrical conductivity after high temperature carbonization. A novel electrochemical sensor based on a N@Fe-MOF@C loaded dual-template molecularly imprinted polymer (DTMIP) modified glassy carbon electrode (GCE) was proposed for the rapid and ultra-sensitive simultaneous detection of clenbuterol hydrochloride (CLB) and ractopamine (RAC). N@Fe-MOF@C combined with a MIP significantly enhanced the electrical signal. Cyclic voltammetry (CV) was used to detect CLB and RAC. The electrochemical polymerization was conducted with O -phenylenediamine as the functional monomer and CLB and RAC as template molecules. The factors affecting the sensor response were optimized. Under the optimal experimental conditions, the CV current response showed a linear range of 10 −12 –8 × 10 −9 M for both CLB and RAC, and the detection limit (LOD) for both CLB and RAC was 3.03 × 10 −13 M (S/N = 3). This electrochemical sensing system has high sensitivity, selectivity, excellent reproducibility, repeatability and stability. The recoveries of the actual samples (97.4%–101.2%) and reasonable relative standard deviations (RSDs) (1.06%–3.17%) indicate the practicability of the sensor system. The system has high application value in the rapid detection of CLB and RAC in clenbuterol hydrochloride tablets, human urine and raw pork.