A novel ratiometric MALDI-MS quantitation strategy for alkaline phosphatase activity with a homogeneous reaction and a tunable dynamic range. Issue 71 (18th August 2021)

• Record Type: Journal Article
• Authors: Ma, Rong-Na; Zhang, Min; Hu, Chao-Long; Pan, Hui-Jing; Si, Lei; Wang, Huaisheng
• Is Part Of: Chemical communications. Volume 57:Issue 71(2021)
• Page Start: 8885
• Page End: 8888
• Publication Date: 2021-08-18
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/d1cc03863j
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : A unique ratiometric MALDI-MS strategy based on a homogeneous enzymatic cleavage triggered double-signal output was designed for the convenient and reliable quantitation of alkaline phosphatase with a tunable dynamic range. Abstract : A unique ratiometric MALDI-MS strategy is proposed for the convenient and reliable quantitation of alkaline phosphatase based on the homogeneous enzymatic cleavage of a coded phosphopeptide (CPP)-triggered double-signal output. The dynamic range can be tuned by simply adjusting the primary concentration of CPP. The proposed strategy is also capable of being challenged by real human serum, and thus it may offer a wonderful approach for the convenient identification and quantitation of various enzyme activities in clinical diagnosis.